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Early embryo development in a sequential versus single medium: a randomized study

BACKGROUND: The success of in vitro fertilization techniques is defined by multiple factors including embryo culture conditions, related to the composition of the culture medium. In view of the lack of solid scientific data and in view of the current general belief that sequential media are superior...

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Autores principales: Paternot, Goedele, Debrock, Sophie, D'Hooghe, Thomas M, Spiessens, Carl
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907384/
https://www.ncbi.nlm.nih.gov/pubmed/20609226
http://dx.doi.org/10.1186/1477-7827-8-83
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author Paternot, Goedele
Debrock, Sophie
D'Hooghe, Thomas M
Spiessens, Carl
author_facet Paternot, Goedele
Debrock, Sophie
D'Hooghe, Thomas M
Spiessens, Carl
author_sort Paternot, Goedele
collection PubMed
description BACKGROUND: The success of in vitro fertilization techniques is defined by multiple factors including embryo culture conditions, related to the composition of the culture medium. In view of the lack of solid scientific data and in view of the current general belief that sequential media are superior to single media, the aim of this randomized study was to compare the embryo quality in two types of culture media. METHODS: In this study, the embryo quality on day 3 was measured as primary outcome. In total, 147 patients younger than 36 years treated with IVF/ICSI during the first or second cycle were included in this study. Embryos were randomly cultured in a sequential (group A) or a single medium (group B) to compare the embryo quality on day 1, day 2 and day 3. The embryo quality was compared in both groups using a Chi-square test with a significance level of 0.05. RESULTS: At day 1, the percentage of embryos with a cytoplasmic halo was higher in group B (46%) than in group A (32%). At day 2, number of blastomeres, degree of fragmentation and the percentage of unequally sized blastomeres were higher in group B than in group A. At day 3, a higher percentage of embryos had a higher number of blastomeres and unequally sized blastomeres in group B. The number of good quality embryos (GQE) was comparable in both groups. The embryo utilization rate was higher in group B (56%) compared to group A (49%). CONCLUSIONS: Although, no significant difference in the number of GQE was found in both media, the utilization rate was significantly higher when the embryos were cultured in the single medium compared to the sequential medium. The results of this study have a possible positive effect on the cumulative cryo-augmented pregnancy rate. TRIAL REGISTRATION NUMBER: NCT01094314
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spelling pubmed-29073842010-07-21 Early embryo development in a sequential versus single medium: a randomized study Paternot, Goedele Debrock, Sophie D'Hooghe, Thomas M Spiessens, Carl Reprod Biol Endocrinol Research BACKGROUND: The success of in vitro fertilization techniques is defined by multiple factors including embryo culture conditions, related to the composition of the culture medium. In view of the lack of solid scientific data and in view of the current general belief that sequential media are superior to single media, the aim of this randomized study was to compare the embryo quality in two types of culture media. METHODS: In this study, the embryo quality on day 3 was measured as primary outcome. In total, 147 patients younger than 36 years treated with IVF/ICSI during the first or second cycle were included in this study. Embryos were randomly cultured in a sequential (group A) or a single medium (group B) to compare the embryo quality on day 1, day 2 and day 3. The embryo quality was compared in both groups using a Chi-square test with a significance level of 0.05. RESULTS: At day 1, the percentage of embryos with a cytoplasmic halo was higher in group B (46%) than in group A (32%). At day 2, number of blastomeres, degree of fragmentation and the percentage of unequally sized blastomeres were higher in group B than in group A. At day 3, a higher percentage of embryos had a higher number of blastomeres and unequally sized blastomeres in group B. The number of good quality embryos (GQE) was comparable in both groups. The embryo utilization rate was higher in group B (56%) compared to group A (49%). CONCLUSIONS: Although, no significant difference in the number of GQE was found in both media, the utilization rate was significantly higher when the embryos were cultured in the single medium compared to the sequential medium. The results of this study have a possible positive effect on the cumulative cryo-augmented pregnancy rate. TRIAL REGISTRATION NUMBER: NCT01094314 BioMed Central 2010-07-07 /pmc/articles/PMC2907384/ /pubmed/20609226 http://dx.doi.org/10.1186/1477-7827-8-83 Text en Copyright ©2010 Paternot et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Paternot, Goedele
Debrock, Sophie
D'Hooghe, Thomas M
Spiessens, Carl
Early embryo development in a sequential versus single medium: a randomized study
title Early embryo development in a sequential versus single medium: a randomized study
title_full Early embryo development in a sequential versus single medium: a randomized study
title_fullStr Early embryo development in a sequential versus single medium: a randomized study
title_full_unstemmed Early embryo development in a sequential versus single medium: a randomized study
title_short Early embryo development in a sequential versus single medium: a randomized study
title_sort early embryo development in a sequential versus single medium: a randomized study
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907384/
https://www.ncbi.nlm.nih.gov/pubmed/20609226
http://dx.doi.org/10.1186/1477-7827-8-83
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