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Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana

Buruli ulcer (BU) is an emerging necrotizing disease of the skin and subcutaneous tissue caused by Mycobacterium ulcerans. While proximity to stagnant or slow flowing water bodies is a risk factor for acquiring BU, the epidemiology and mode of M. ulcerans transmission is poorly understood. Here we h...

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Autores principales: Röltgen, Katharina, Qi, Weihong, Ruf, Marie-Thérèse, Mensah-Quainoo, Ernestina, Pidot, Sacha J., Seemann, Torsten, Stinear, Timothy P., Käser, Michael, Yeboah-Manu, Dorothy, Pluschke, Gerd
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907412/
https://www.ncbi.nlm.nih.gov/pubmed/20652033
http://dx.doi.org/10.1371/journal.pntd.0000751
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author Röltgen, Katharina
Qi, Weihong
Ruf, Marie-Thérèse
Mensah-Quainoo, Ernestina
Pidot, Sacha J.
Seemann, Torsten
Stinear, Timothy P.
Käser, Michael
Yeboah-Manu, Dorothy
Pluschke, Gerd
author_facet Röltgen, Katharina
Qi, Weihong
Ruf, Marie-Thérèse
Mensah-Quainoo, Ernestina
Pidot, Sacha J.
Seemann, Torsten
Stinear, Timothy P.
Käser, Michael
Yeboah-Manu, Dorothy
Pluschke, Gerd
author_sort Röltgen, Katharina
collection PubMed
description Buruli ulcer (BU) is an emerging necrotizing disease of the skin and subcutaneous tissue caused by Mycobacterium ulcerans. While proximity to stagnant or slow flowing water bodies is a risk factor for acquiring BU, the epidemiology and mode of M. ulcerans transmission is poorly understood. Here we have used high-throughput DNA sequencing and comparisons of the genomes of seven M. ulcerans isolates that appeared monomorphic by existing typing methods. We identified a limited number of single nucleotide polymorphisms (SNPs) and developed a real-time PCR SNP typing method based on these differences. We then investigated clinical isolates of M. ulcerans on which we had detailed information concerning patient location and time of diagnosis. Within the Densu river basin of Ghana we observed dominance of one clonal complex and local clustering of some of the variants belonging to this complex. These results reveal focal transmission and demonstrate, that micro-epidemiological analyses by SNP typing has great potential to help us understand how M. ulcerans is transmitted.
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spelling pubmed-29074122010-07-22 Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana Röltgen, Katharina Qi, Weihong Ruf, Marie-Thérèse Mensah-Quainoo, Ernestina Pidot, Sacha J. Seemann, Torsten Stinear, Timothy P. Käser, Michael Yeboah-Manu, Dorothy Pluschke, Gerd PLoS Negl Trop Dis Research Article Buruli ulcer (BU) is an emerging necrotizing disease of the skin and subcutaneous tissue caused by Mycobacterium ulcerans. While proximity to stagnant or slow flowing water bodies is a risk factor for acquiring BU, the epidemiology and mode of M. ulcerans transmission is poorly understood. Here we have used high-throughput DNA sequencing and comparisons of the genomes of seven M. ulcerans isolates that appeared monomorphic by existing typing methods. We identified a limited number of single nucleotide polymorphisms (SNPs) and developed a real-time PCR SNP typing method based on these differences. We then investigated clinical isolates of M. ulcerans on which we had detailed information concerning patient location and time of diagnosis. Within the Densu river basin of Ghana we observed dominance of one clonal complex and local clustering of some of the variants belonging to this complex. These results reveal focal transmission and demonstrate, that micro-epidemiological analyses by SNP typing has great potential to help us understand how M. ulcerans is transmitted. Public Library of Science 2010-07-20 /pmc/articles/PMC2907412/ /pubmed/20652033 http://dx.doi.org/10.1371/journal.pntd.0000751 Text en Röltgen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Röltgen, Katharina
Qi, Weihong
Ruf, Marie-Thérèse
Mensah-Quainoo, Ernestina
Pidot, Sacha J.
Seemann, Torsten
Stinear, Timothy P.
Käser, Michael
Yeboah-Manu, Dorothy
Pluschke, Gerd
Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana
title Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana
title_full Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana
title_fullStr Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana
title_full_unstemmed Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana
title_short Single Nucleotide Polymorphism Typing of Mycobacterium ulcerans Reveals Focal Transmission of Buruli Ulcer in a Highly Endemic Region of Ghana
title_sort single nucleotide polymorphism typing of mycobacterium ulcerans reveals focal transmission of buruli ulcer in a highly endemic region of ghana
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907412/
https://www.ncbi.nlm.nih.gov/pubmed/20652033
http://dx.doi.org/10.1371/journal.pntd.0000751
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