A Strategy for the Selective Imaging of Glycans Using Caged Metabolic Precursors

[Image: see text] Glycans can be imaged by metabolic labeling with azidosugars followed by chemical reaction with imaging probes; however, tissue-specific labeling is difficult to achieve. Here we describe a strategy for the use of a caged metabolic precursor that is activated for cellular metabolis...

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Autores principales: Chang, Pamela V., Dube, Danielle H., Sletten, Ellen M., Bertozzi, Carolyn R.
Formato: Texto
Lenguaje:English
Publicado: American Chemical Society 2010
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907715/
https://www.ncbi.nlm.nih.gov/pubmed/20568764
http://dx.doi.org/10.1021/ja101080y
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author Chang, Pamela V.
Dube, Danielle H.
Sletten, Ellen M.
Bertozzi, Carolyn R.
author_facet Chang, Pamela V.
Dube, Danielle H.
Sletten, Ellen M.
Bertozzi, Carolyn R.
author_sort Chang, Pamela V.
collection PubMed
description [Image: see text] Glycans can be imaged by metabolic labeling with azidosugars followed by chemical reaction with imaging probes; however, tissue-specific labeling is difficult to achieve. Here we describe a strategy for the use of a caged metabolic precursor that is activated for cellular metabolism by enzymatic cleavage. An N-azidoacetylmannosamine derivative caged with a peptide substrate for the prostate-specific antigen (PSA) protease was converted to cell-surface azido sialic acids in a PSA-dependent manner. The approach has applications in tissue-selective imaging of glycans for clinical and basic research purposes.
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spelling pubmed-29077152010-07-21 A Strategy for the Selective Imaging of Glycans Using Caged Metabolic Precursors Chang, Pamela V. Dube, Danielle H. Sletten, Ellen M. Bertozzi, Carolyn R. J Am Chem Soc [Image: see text] Glycans can be imaged by metabolic labeling with azidosugars followed by chemical reaction with imaging probes; however, tissue-specific labeling is difficult to achieve. Here we describe a strategy for the use of a caged metabolic precursor that is activated for cellular metabolism by enzymatic cleavage. An N-azidoacetylmannosamine derivative caged with a peptide substrate for the prostate-specific antigen (PSA) protease was converted to cell-surface azido sialic acids in a PSA-dependent manner. The approach has applications in tissue-selective imaging of glycans for clinical and basic research purposes. American Chemical Society 2010-06-22 2010-07-21 /pmc/articles/PMC2907715/ /pubmed/20568764 http://dx.doi.org/10.1021/ja101080y Text en Copyright © 2010 American Chemical Society http://pubs.acs.org This is an open-access article distributed under the ACS AuthorChoice Terms & Conditions. Any use of this article, must conform to the terms of that license which are available at http://pubs.acs.org.
spellingShingle Chang, Pamela V.
Dube, Danielle H.
Sletten, Ellen M.
Bertozzi, Carolyn R.
A Strategy for the Selective Imaging of Glycans Using Caged Metabolic Precursors
title A Strategy for the Selective Imaging of Glycans Using Caged Metabolic Precursors
title_full A Strategy for the Selective Imaging of Glycans Using Caged Metabolic Precursors
title_fullStr A Strategy for the Selective Imaging of Glycans Using Caged Metabolic Precursors
title_full_unstemmed A Strategy for the Selective Imaging of Glycans Using Caged Metabolic Precursors
title_short A Strategy for the Selective Imaging of Glycans Using Caged Metabolic Precursors
title_sort strategy for the selective imaging of glycans using caged metabolic precursors
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2907715/
https://www.ncbi.nlm.nih.gov/pubmed/20568764
http://dx.doi.org/10.1021/ja101080y
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