Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing

BACKGROUND: OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality. RESULTS: We can assess performance...

Descripción completa

Detalles Bibliográficos
Autores principales: Azulay, David-Olivier D, Neubert, Hendrik, Ocaña, Mireia Fernández
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908104/
https://www.ncbi.nlm.nih.gov/pubmed/20602788
http://dx.doi.org/10.1186/1471-2105-11-371
Descripción
Sumario:BACKGROUND: OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality. RESULTS: We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions. CONCLUSIONS: A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research.

Ejemplares similares