Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing

BACKGROUND: OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality. RESULTS: We can assess performance...

Descripción completa

Detalles Bibliográficos
Autores principales: Azulay, David-Olivier D, Neubert, Hendrik, Ocaña, Mireia Fernández
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Software
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908104/
https://www.ncbi.nlm.nih.gov/pubmed/20602788
http://dx.doi.org/10.1186/1471-2105-11-371
_version_ 1782184156515532800
author Azulay, David-Olivier D
Neubert, Hendrik
Ocaña, Mireia Fernández
author_facet Azulay, David-Olivier D
Neubert, Hendrik
Ocaña, Mireia Fernández
author_sort Azulay, David-Olivier D
collection PubMed
description BACKGROUND: OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality. RESULTS: We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions. CONCLUSIONS: A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research.
format Text
id pubmed-2908104
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-29081042010-07-22 Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing Azulay, David-Olivier D Neubert, Hendrik Ocaña, Mireia Fernández BMC Bioinformatics Software BACKGROUND: OFFGEL isoelectric focussing (IEF) has become a popular tool in proteomics to fractionate peptides or proteins. As a consequence there is a need for software solutions supporting data mining, interpretation and characterisation of experimental quality. RESULTS: We can assess performance characteristics of OFFGEL IEF peptide fractionation in proteomics by generating plots of the overall fractionation patterns and the pairwise comparisons of adjacent fractions. CONCLUSIONS: A visualisation tool for peptide fractionation has been developed to support the evaluation of IEF data quality and can be implemented in proteomics research. BioMed Central 2010-07-05 /pmc/articles/PMC2908104/ /pubmed/20602788 http://dx.doi.org/10.1186/1471-2105-11-371 Text en Copyright ©2010 Azulay et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Software
Azulay, David-Olivier D
Neubert, Hendrik
Ocaña, Mireia Fernández
Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
title Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
title_full Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
title_fullStr Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
title_full_unstemmed Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
title_short Visualisation tool for peptide fractionation data in proteomics: application to OFFGEL isoelectric focussing
title_sort visualisation tool for peptide fractionation data in proteomics: application to offgel isoelectric focussing
topic Software
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908104/
https://www.ncbi.nlm.nih.gov/pubmed/20602788
http://dx.doi.org/10.1186/1471-2105-11-371
work_keys_str_mv AT azulaydavidolivierd visualisationtoolforpeptidefractionationdatainproteomicsapplicationtooffgelisoelectricfocussing
AT neuberthendrik visualisationtoolforpeptidefractionationdatainproteomicsapplicationtooffgelisoelectricfocussing
AT ocanamireiafernandez visualisationtoolforpeptidefractionationdatainproteomicsapplicationtooffgelisoelectricfocussing

Ejemplares similares