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Uropathogenic Escherichia coli Modulates Immune Responses and Its Curli Fimbriae Interact with the Antimicrobial Peptide LL-37
Bacterial growth in multicellular communities, or biofilms, offers many potential advantages over single-cell growth, including resistance to antimicrobial factors. Here we describe the interaction between the biofilm-promoting components curli fimbriae and cellulose of uropathogenic E. coli and the...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908543/ https://www.ncbi.nlm.nih.gov/pubmed/20661475 http://dx.doi.org/10.1371/journal.ppat.1001010 |
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author | Kai-Larsen, Ylva Lüthje, Petra Chromek, Milan Peters, Verena Wang, Xiaoda Holm, Åsa Kádas, Lavinia Hedlund, Kjell-Olof Johansson, Jan Chapman, Matthew R. Jacobson, Stefan H. Römling, Ute Agerberth, Birgitta Brauner, Annelie |
author_facet | Kai-Larsen, Ylva Lüthje, Petra Chromek, Milan Peters, Verena Wang, Xiaoda Holm, Åsa Kádas, Lavinia Hedlund, Kjell-Olof Johansson, Jan Chapman, Matthew R. Jacobson, Stefan H. Römling, Ute Agerberth, Birgitta Brauner, Annelie |
author_sort | Kai-Larsen, Ylva |
collection | PubMed |
description | Bacterial growth in multicellular communities, or biofilms, offers many potential advantages over single-cell growth, including resistance to antimicrobial factors. Here we describe the interaction between the biofilm-promoting components curli fimbriae and cellulose of uropathogenic E. coli and the endogenous antimicrobial defense in the urinary tract. We also demonstrate the impact of this interplay on the pathogenesis of urinary tract infections. Our results suggest that curli and cellulose exhibit differential and complementary functions. Both of these biofilm components were expressed by a high proportion of clinical E. coli isolates. Curli promoted adherence to epithelial cells and resistance against the human antimicrobial peptide LL-37, but also increased the induction of the proinflammatory cytokine IL-8. Cellulose production, on the other hand, reduced immune induction and hence delayed bacterial elimination from the kidneys. Interestingly, LL-37 inhibited curli formation by preventing the polymerization of the major curli subunit, CsgA. Thus, even relatively low concentrations of LL-37 inhibited curli-mediated biofilm formation in vitro. Taken together, our data demonstrate that biofilm components are involved in the pathogenesis of urinary tract infections by E. coli and can be a target of local immune defense mechanisms. |
format | Text |
id | pubmed-2908543 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29085432010-07-26 Uropathogenic Escherichia coli Modulates Immune Responses and Its Curli Fimbriae Interact with the Antimicrobial Peptide LL-37 Kai-Larsen, Ylva Lüthje, Petra Chromek, Milan Peters, Verena Wang, Xiaoda Holm, Åsa Kádas, Lavinia Hedlund, Kjell-Olof Johansson, Jan Chapman, Matthew R. Jacobson, Stefan H. Römling, Ute Agerberth, Birgitta Brauner, Annelie PLoS Pathog Research Article Bacterial growth in multicellular communities, or biofilms, offers many potential advantages over single-cell growth, including resistance to antimicrobial factors. Here we describe the interaction between the biofilm-promoting components curli fimbriae and cellulose of uropathogenic E. coli and the endogenous antimicrobial defense in the urinary tract. We also demonstrate the impact of this interplay on the pathogenesis of urinary tract infections. Our results suggest that curli and cellulose exhibit differential and complementary functions. Both of these biofilm components were expressed by a high proportion of clinical E. coli isolates. Curli promoted adherence to epithelial cells and resistance against the human antimicrobial peptide LL-37, but also increased the induction of the proinflammatory cytokine IL-8. Cellulose production, on the other hand, reduced immune induction and hence delayed bacterial elimination from the kidneys. Interestingly, LL-37 inhibited curli formation by preventing the polymerization of the major curli subunit, CsgA. Thus, even relatively low concentrations of LL-37 inhibited curli-mediated biofilm formation in vitro. Taken together, our data demonstrate that biofilm components are involved in the pathogenesis of urinary tract infections by E. coli and can be a target of local immune defense mechanisms. Public Library of Science 2010-07-22 /pmc/articles/PMC2908543/ /pubmed/20661475 http://dx.doi.org/10.1371/journal.ppat.1001010 Text en Kai-Larsen et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kai-Larsen, Ylva Lüthje, Petra Chromek, Milan Peters, Verena Wang, Xiaoda Holm, Åsa Kádas, Lavinia Hedlund, Kjell-Olof Johansson, Jan Chapman, Matthew R. Jacobson, Stefan H. Römling, Ute Agerberth, Birgitta Brauner, Annelie Uropathogenic Escherichia coli Modulates Immune Responses and Its Curli Fimbriae Interact with the Antimicrobial Peptide LL-37 |
title | Uropathogenic Escherichia coli Modulates Immune Responses and Its Curli Fimbriae Interact with the Antimicrobial Peptide LL-37 |
title_full | Uropathogenic Escherichia coli Modulates Immune Responses and Its Curli Fimbriae Interact with the Antimicrobial Peptide LL-37 |
title_fullStr | Uropathogenic Escherichia coli Modulates Immune Responses and Its Curli Fimbriae Interact with the Antimicrobial Peptide LL-37 |
title_full_unstemmed | Uropathogenic Escherichia coli Modulates Immune Responses and Its Curli Fimbriae Interact with the Antimicrobial Peptide LL-37 |
title_short | Uropathogenic Escherichia coli Modulates Immune Responses and Its Curli Fimbriae Interact with the Antimicrobial Peptide LL-37 |
title_sort | uropathogenic escherichia coli modulates immune responses and its curli fimbriae interact with the antimicrobial peptide ll-37 |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908543/ https://www.ncbi.nlm.nih.gov/pubmed/20661475 http://dx.doi.org/10.1371/journal.ppat.1001010 |
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