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Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus

BACKGROUND: Betanodaviruses, members of the family Nodaviridae, have bipartite, positive-sense RNA genomes and are the causal agents of viral nervous necrosis in many marine fish species. Recently, the viruses were shown to infect a few freshwater fish species including a model fish medaka (Oryzias...

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Autores principales: Adachi, Kei, Sumiyoshi, Kosuke, Ariyasu, Ryo, Yamashita, Kasumi, Zenke, Kosuke, Okinaka, Yasushi
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908575/
https://www.ncbi.nlm.nih.gov/pubmed/20624282
http://dx.doi.org/10.1186/1743-422X-7-150
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author Adachi, Kei
Sumiyoshi, Kosuke
Ariyasu, Ryo
Yamashita, Kasumi
Zenke, Kosuke
Okinaka, Yasushi
author_facet Adachi, Kei
Sumiyoshi, Kosuke
Ariyasu, Ryo
Yamashita, Kasumi
Zenke, Kosuke
Okinaka, Yasushi
author_sort Adachi, Kei
collection PubMed
description BACKGROUND: Betanodaviruses, members of the family Nodaviridae, have bipartite, positive-sense RNA genomes and are the causal agents of viral nervous necrosis in many marine fish species. Recently, the viruses were shown to infect a few freshwater fish species including a model fish medaka (Oryzias latipes). Although virological study using cultured medaka cells would provide a lot of insight into virus-fish interactions in molecular aspects, no such cells have yet been tested for virus susceptibility. RESULTS: We tested ten medaka cell lines for susceptibilities to redspotted grouper nervous necrosis virus (RGNNV). Although the viral coat protein was detected in all the cell lines inoculated, the levels of cytopathic effect development and viral propagation were quite different among the cell lines. Those levels were especially high in OLHNI-1 and OLHNI-2 cells, but were extremely low in OLME-104 cells. Some cell lines entered into antiviral state after RGNNV infections probably because of inducing an antiviral system. This is the first report to examine the susceptibilities of cultured medaka cells against a virus. CONCLUSION: OLHNI-1 and OLHNI-2 cells are candidates of new standard cells for betanodavirus study because of their high susceptibilities to the virus and their several advantages as model fish cells.
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spelling pubmed-29085752010-07-23 Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus Adachi, Kei Sumiyoshi, Kosuke Ariyasu, Ryo Yamashita, Kasumi Zenke, Kosuke Okinaka, Yasushi Virol J Research BACKGROUND: Betanodaviruses, members of the family Nodaviridae, have bipartite, positive-sense RNA genomes and are the causal agents of viral nervous necrosis in many marine fish species. Recently, the viruses were shown to infect a few freshwater fish species including a model fish medaka (Oryzias latipes). Although virological study using cultured medaka cells would provide a lot of insight into virus-fish interactions in molecular aspects, no such cells have yet been tested for virus susceptibility. RESULTS: We tested ten medaka cell lines for susceptibilities to redspotted grouper nervous necrosis virus (RGNNV). Although the viral coat protein was detected in all the cell lines inoculated, the levels of cytopathic effect development and viral propagation were quite different among the cell lines. Those levels were especially high in OLHNI-1 and OLHNI-2 cells, but were extremely low in OLME-104 cells. Some cell lines entered into antiviral state after RGNNV infections probably because of inducing an antiviral system. This is the first report to examine the susceptibilities of cultured medaka cells against a virus. CONCLUSION: OLHNI-1 and OLHNI-2 cells are candidates of new standard cells for betanodavirus study because of their high susceptibilities to the virus and their several advantages as model fish cells. BioMed Central 2010-07-12 /pmc/articles/PMC2908575/ /pubmed/20624282 http://dx.doi.org/10.1186/1743-422X-7-150 Text en Copyright ©2010 Adachi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Adachi, Kei
Sumiyoshi, Kosuke
Ariyasu, Ryo
Yamashita, Kasumi
Zenke, Kosuke
Okinaka, Yasushi
Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus
title Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus
title_full Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus
title_fullStr Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus
title_full_unstemmed Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus
title_short Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus
title_sort susceptibilities of medaka (oryzias latipes) cell lines to a betanodavirus
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2908575/
https://www.ncbi.nlm.nih.gov/pubmed/20624282
http://dx.doi.org/10.1186/1743-422X-7-150
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