A simple method for generating full length cDNA from low abundance partial genomic clones

BACKGROUND: PCR amplification of target molecules involves sequence specific primers that flank the region to be amplified. While this technique is generally routine, its applicability may not be sufficient to generate a desired target molecule from two separate regions involving intron /exon bounda...

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Detalles Bibliográficos
Autores principales: Wang, Yongxin, Fugaro, Joseph M, Siddiq, Fauzia, Goparaju, Chandra Mouli V, Lonardo, Fulvio, Wali, Anil, Lechner, John F, Pass, Harvey I
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2000
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC29089/
https://www.ncbi.nlm.nih.gov/pubmed/11114844
http://dx.doi.org/10.1186/1471-2199-1-2

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