Snapshot Image Mapping Spectrometer (IMS) with high sampling density for hyperspectral microscopy

A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 × 285 × 60 (x, y, λ). The spatial resolution is ~0.45 µm with a FOV of 100 × 100 µm(2). The measured spectrum is from 450 nm to 650 nm and is sampl...

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Detalles Bibliográficos
Autores principales: Gao, Liang, Kester, Robert T., Hagen, Nathan, Tkaczyk, Tomasz S.
Formato: Texto
Lenguaje:English
Publicado: Optical Society of America 2010
Materias:
Research-Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2909105/
https://www.ncbi.nlm.nih.gov/pubmed/20639917
http://dx.doi.org/10.1364/OE.18.014330
Descripción
Sumario:A snapshot Image Mapping Spectrometer (IMS) with high sampling density is developed for hyperspectral microscopy, measuring a datacube of dimensions 285 × 285 × 60 (x, y, λ). The spatial resolution is ~0.45 µm with a FOV of 100 × 100 µm(2). The measured spectrum is from 450 nm to 650 nm and is sampled by 60 spectral channels with average sampling interval ~3.3 nm. The channel’s spectral resolution is ~8nm. The spectral imaging results demonstrate the potential of the IMS for real-time cellular fluorescence imaging.

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