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Ironing out the wrinkles in the rare biosphere through improved OTU clustering
Deep sequencing of PCR amplicon libraries facilitates the detection of low-abundance populations in environmental DNA surveys of complex microbial communities. At the same time, deep sequencing can lead to overestimates of microbial diversity through the generation of low-frequency, error-prone read...
Autores principales: | Huse, Susan M, Welch, David Mark, Morrison, Hilary G, Sogin, Mitchell L |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Blackwell Publishing Ltd
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2909393/ https://www.ncbi.nlm.nih.gov/pubmed/20236171 http://dx.doi.org/10.1111/j.1462-2920.2010.02193.x |
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