Cargando…
Identification and characterization of the two isoforms of the vertebrate H2A.Z histone variant
Histone variants play important roles in the epigenetic regulation of genome function. The histone variant H2A.Z is evolutionarily conserved from yeast to vertebrates, and it has been reported to have multiple effects upon gene expression and insulation, and chromosome segregation. Recently two gene...
Autores principales: | , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910051/ https://www.ncbi.nlm.nih.gov/pubmed/20299344 http://dx.doi.org/10.1093/nar/gkq171 |
_version_ | 1782184359414988800 |
---|---|
author | Matsuda, Ryo Hori, Tetsuya Kitamura, Hiroshi Takeuchi, Kozo Fukagawa, Tatsuo Harata, Masahiko |
author_facet | Matsuda, Ryo Hori, Tetsuya Kitamura, Hiroshi Takeuchi, Kozo Fukagawa, Tatsuo Harata, Masahiko |
author_sort | Matsuda, Ryo |
collection | PubMed |
description | Histone variants play important roles in the epigenetic regulation of genome function. The histone variant H2A.Z is evolutionarily conserved from yeast to vertebrates, and it has been reported to have multiple effects upon gene expression and insulation, and chromosome segregation. Recently two genes encoding H2A.Z were identified in the vertebrate genome. However, it is not yet clear whether the proteins transcribed from these genes are functionally distinct. To address this issue, we knocked out each gene individually in chicken DT40 cells. We found that two distinct proteins, H2A.Z-1 and H2A.Z-2, were produced from these genes, and that these proteins could be separated on a long SDS–PAGE gel. The two isoforms were deposited to a similar extent by the SRCAP chromatin-remodeling complex, suggesting redundancy to their function. However, cells lacking either one of the two isoforms exhibited distinct alterations in cell growth and gene expression, suggesting that the two isoforms have differential effects upon nucleosome stability and chromatin structure. These findings provide insight into the molecular basis of the multiple functions of the H2A.Z gene products. |
format | Text |
id | pubmed-2910051 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-29100512010-07-27 Identification and characterization of the two isoforms of the vertebrate H2A.Z histone variant Matsuda, Ryo Hori, Tetsuya Kitamura, Hiroshi Takeuchi, Kozo Fukagawa, Tatsuo Harata, Masahiko Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics Histone variants play important roles in the epigenetic regulation of genome function. The histone variant H2A.Z is evolutionarily conserved from yeast to vertebrates, and it has been reported to have multiple effects upon gene expression and insulation, and chromosome segregation. Recently two genes encoding H2A.Z were identified in the vertebrate genome. However, it is not yet clear whether the proteins transcribed from these genes are functionally distinct. To address this issue, we knocked out each gene individually in chicken DT40 cells. We found that two distinct proteins, H2A.Z-1 and H2A.Z-2, were produced from these genes, and that these proteins could be separated on a long SDS–PAGE gel. The two isoforms were deposited to a similar extent by the SRCAP chromatin-remodeling complex, suggesting redundancy to their function. However, cells lacking either one of the two isoforms exhibited distinct alterations in cell growth and gene expression, suggesting that the two isoforms have differential effects upon nucleosome stability and chromatin structure. These findings provide insight into the molecular basis of the multiple functions of the H2A.Z gene products. Oxford University Press 2010-07 2010-03-18 /pmc/articles/PMC2910051/ /pubmed/20299344 http://dx.doi.org/10.1093/nar/gkq171 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Gene Regulation, Chromatin and Epigenetics Matsuda, Ryo Hori, Tetsuya Kitamura, Hiroshi Takeuchi, Kozo Fukagawa, Tatsuo Harata, Masahiko Identification and characterization of the two isoforms of the vertebrate H2A.Z histone variant |
title | Identification and characterization of the two isoforms of the vertebrate H2A.Z histone variant |
title_full | Identification and characterization of the two isoforms of the vertebrate H2A.Z histone variant |
title_fullStr | Identification and characterization of the two isoforms of the vertebrate H2A.Z histone variant |
title_full_unstemmed | Identification and characterization of the two isoforms of the vertebrate H2A.Z histone variant |
title_short | Identification and characterization of the two isoforms of the vertebrate H2A.Z histone variant |
title_sort | identification and characterization of the two isoforms of the vertebrate h2a.z histone variant |
topic | Gene Regulation, Chromatin and Epigenetics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910051/ https://www.ncbi.nlm.nih.gov/pubmed/20299344 http://dx.doi.org/10.1093/nar/gkq171 |
work_keys_str_mv | AT matsudaryo identificationandcharacterizationofthetwoisoformsofthevertebrateh2azhistonevariant AT horitetsuya identificationandcharacterizationofthetwoisoformsofthevertebrateh2azhistonevariant AT kitamurahiroshi identificationandcharacterizationofthetwoisoformsofthevertebrateh2azhistonevariant AT takeuchikozo identificationandcharacterizationofthetwoisoformsofthevertebrateh2azhistonevariant AT fukagawatatsuo identificationandcharacterizationofthetwoisoformsofthevertebrateh2azhistonevariant AT haratamasahiko identificationandcharacterizationofthetwoisoformsofthevertebrateh2azhistonevariant |