Cargando…

Synergistic effects between analogs of DNA and RNA improve the potency of siRNA-mediated gene silencing

We report that combining a DNA analog (2′F-ANA) with rigid RNA analogs [2′F-RNA and/or locked nucleic acid (LNA)] in siRNA duplexes can produce gene silencing agents with enhanced potency. The favored conformations of these two analogs are different, and combining them in a 1–1 pattern led to reduce...

Descripción completa

Detalles Bibliográficos
Autores principales:	Deleavey, Glen F., Watts, Jonathan K., Alain, Tommy, Robert, Francis, Kalota, Anna, Aishwarya, Veenu, Pelletier, Jerry, Gewirtz, Alan M., Sonenberg, Nahum, Damha, Masad J.
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2010
Materias:	Synthetic Biology and Chemistry
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910058/ https://www.ncbi.nlm.nih.gov/pubmed/20413581 http://dx.doi.org/10.1093/nar/gkq181

Ejemplares similares

Modulation of thermal stability can enhance the potency of siRNA
por: Addepalli, Haripriya, et al.
Publicado: (2010)

Antisense 2′-Deoxy, 2′-Fluoroarabino Nucleic Acid (2′F-ANA) Oligonucleotides: In Vitro Gymnotic Silencers of Gene Expression Whose Potency Is Enhanced by Fatty Acids
por: Souleimanian, Naira, et al.
Publicado: (2012)

2′-Fluoro-4′-thioarabino-modified oligonucleotides: conformational switches linked to siRNA activity
por: Watts, Jonathan K., et al.
Publicado: (2007)

Effect of Sugar 2′,4′-Modifications on Gene Silencing Activity of siRNA Duplexes
por: Malek-Adamian, Elise, et al.
Publicado: (2019)

Carrier-free cellular uptake and the gene-silencing activity of the lipophilic siRNAs is strongly affected by the length of the linker between siRNA and lipophilic group
por: Petrova, Natalya S., et al.
Publicado: (2012)

High potency silencing by single-stranded boranophosphate siRNA
por: Hall, Allison H. S., et al.
Publicado: (2006)

A single-label phenylpyrrolocytidine provides a molecular beacon-like response reporting HIV-1 RT RNase H activity
por: Wahba, Alexander S., et al.
Publicado: (2010)

Maximizing the Potency of siRNA Lipid Nanoparticles for Hepatic Gene Silencing In Vivo**
por: Jayaraman, Muthusamy, et al.
Publicado: (2012)

ss-siRNAs allele selectively inhibit ataxin-3 expression: multiple mechanisms for an alternative gene silencing strategy
por: Liu, Jing, et al.
Publicado: (2013)

2′-Deoxy-2′-fluoro-β-d-arabinonucleic acid (2′F-ANA) modified oligonucleotides (ON) effect highly efficient, and persistent, gene silencing
por: Kalota, A., et al.
Publicado: (2006)

Substrate analogs that trap the 2′-phospho-ADP-ribosylated RNA intermediate of the Tpt1 (tRNA 2′-phosphotransferase) reaction pathway
por: Dantuluri, Swathi, et al.
Publicado: (2020)

Light-activated RNA interference using double-stranded siRNA precursors modified using a remarkable regiospecificity of diazo-based photolabile groups
por: Shah, Samit, et al.
Publicado: (2009)

Improvements in siRNA properties mediated by 2′-deoxy-2′-fluoro-β-d-arabinonucleic acid (FANA)
por: Dowler, Thomas, et al.
Publicado: (2006)

CRISPR/Cas9-mediated gene knockout is insensitive to target copy number but is dependent on guide RNA potency and Cas9/sgRNA threshold expression level
por: Yuen, Garmen, et al.
Publicado: (2017)

Intracellular stability of 2′-OMe-4′-thioribonucleoside modified siRNA leads to long-term RNAi effect
por: Takahashi, Mayumi, et al.
Publicado: (2012)

Design of a peptide-based vector, PepFect6, for efficient delivery of siRNA in cell culture and systemically in vivo
por: EL Andaloussi, Samir, et al.
Publicado: (2011)

Dual Mechanisms of Action of Self-Delivering, Anti-HIV-1 FANA Oligonucleotides as a Potential New Approach to HIV Therapy
por: Takahashi, Mayumi, et al.
Publicado: (2019)

A systematic study on the influence of thermodynamic asymmetry of 5′-ends of siRNA duplexes in relation to their silencing potency
por: Lisowiec-Wąchnicka, Jolanta, et al.
Publicado: (2019)

Synthesis, properties, and biological activity of boranophosphate analogs of the mRNA cap: versatile tools for manipulation of therapeutically relevant cap-dependent processes
por: Kowalska, Joanna, et al.
Publicado: (2014)

Aromatic N versus aromatic F: bioisosterism discovered in RNA base pairing interactions leads to a novel class of universal base analogs
por: Koller, Alrun N., et al.
Publicado: (2010)

Competition for RISC binding predicts in vitro potency of siRNA
por: Koller, Erich, et al.
Publicado: (2006)

The eukaryotic initiation factor eIF4H facilitates loop-binding, repetitive RNA unwinding by the eIF4A DEAD-box helicase
por: Sun, Yingjie, et al.
Publicado: (2012)

Therapeutic siRNA silencing in inflammatory monocytes
por: Leuschner, Florian, et al.
Publicado: (2011)

siRNA Versus miRNA as Therapeutics for Gene Silencing
por: Lam, Jenny K W, et al.
Publicado: (2015)

siRNA potency enhancement via chemical modifications of nucleotide bases at the 5′-end of the siRNA guide strand
por: Shinohara, Fumikazu, et al.
Publicado: (2021)

The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing
por: Herbert, Mike, et al.
Publicado: (2011)

Predicting siRNA potency with random forests and support vector machines
por: Wang, Liangjiang, et al.
Publicado: (2010)

Nanocarriers for delivery of siRNA as gene silencing mediator
por: Morales-Becerril, Aideé, et al.
Publicado: (2022)

High‐Density RNA Microarrays Synthesized In Situ by Photolithography
por: Lietard, Jory, et al.
Publicado: (2018)

Insight into the relationship between the cell culture model, cell trafficking and siRNA silencing efficiency
por: Capel, Victoria, et al.
Publicado: (2016)

Structural properties and gene-silencing activity of chemically modified DNA–RNA hybrids with parallel orientation
por: Habibian, Maryam, et al.
Publicado: (2018)

Probing the active site tryptophan of Staphylococcus aureus thioredoxin with an analog
por: Englert, Markus, et al.
Publicado: (2015)

Human DDX6 effects miRNA-mediated gene silencing via direct binding to CNOT1
por: Rouya, Christopher, et al.
Publicado: (2014)

Large-Scale Photolithographic Synthesis of Chimeric DNA/RNA Hairpin Microarrays To Explore Sequence Specificity Landscapes of RNase HII Cleavage
por: Lietard, Jory, et al.
Publicado: (2019)

Programmable RNA base editing with a single gRNA-free enzyme
por: Han, Wenjian, et al.
Publicado: (2022)

Strand antagonism in RNAi: an explanation of differences in potency between intracellularly expressed siRNA and shRNA
por: Jin, Xin, et al.
Publicado: (2012)

Strand antagonism in RNAi: an explanation of differences in potency between intracellularly expressed siRNA and shRNA
por: Jin, Xin, et al.
Publicado: (2020)

Improved specificity of gene silencing by siRNAs containing unlocked nucleobase analogs
por: Vaish, Narendra, et al.
Publicado: (2011)

Properties of pseudo-complementary DNA substituted with weakly pairing analogs of guanine or cytosine
por: Lahoud, Georges, et al.
Publicado: (2008)

Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells
por: Alshehri, Abdullah, et al.
Publicado: (2018)

Cannot write session to /tmp/vufind_sessions/sess_falno9angvkr63fq9s14sk2baq