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Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging

The peak signal or the signal at a predetermined, fixed time point after D-luciferin injection may be used for the quantitative analysis of in vivo bioluminescence imaging. We repeatedly performed sequential bioluminescence imaging after subcutaneous injection of D-luciferin in mice bearing subcutan...

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Autores principales: Inoue, Yusuke, Kiryu, Shigeru, Watanabe, Makoto, Tojo, Arinobu, Ohtomo, Kuni
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910476/
https://www.ncbi.nlm.nih.gov/pubmed/20671955
http://dx.doi.org/10.1155/2010/471408
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author Inoue, Yusuke
Kiryu, Shigeru
Watanabe, Makoto
Tojo, Arinobu
Ohtomo, Kuni
author_facet Inoue, Yusuke
Kiryu, Shigeru
Watanabe, Makoto
Tojo, Arinobu
Ohtomo, Kuni
author_sort Inoue, Yusuke
collection PubMed
description The peak signal or the signal at a predetermined, fixed time point after D-luciferin injection may be used for the quantitative analysis of in vivo bioluminescence imaging. We repeatedly performed sequential bioluminescence imaging after subcutaneous injection of D-luciferin in mice bearing subcutaneous tumors. The peak time in each measurement became shorter early after cell inoculation, presumably due to gradual establishment of intratumoral vasculature, and reached a plateau of about 10 min on day 10. Although the correlation between the signal at a fixed time point and the peak signal was high, the signal at 5 or 10 min normalized for the peak signal was lower for earlier days, which caused overestimation of tumor growth. The time course of the signals after D-luciferin injection may vary with time after cell inoculation, and this variation should be considered when determining the imaging protocol for quantitative bioluminescence tumor monitoring.
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spelling pubmed-29104762010-07-29 Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging Inoue, Yusuke Kiryu, Shigeru Watanabe, Makoto Tojo, Arinobu Ohtomo, Kuni Int J Biomed Imaging Research Article The peak signal or the signal at a predetermined, fixed time point after D-luciferin injection may be used for the quantitative analysis of in vivo bioluminescence imaging. We repeatedly performed sequential bioluminescence imaging after subcutaneous injection of D-luciferin in mice bearing subcutaneous tumors. The peak time in each measurement became shorter early after cell inoculation, presumably due to gradual establishment of intratumoral vasculature, and reached a plateau of about 10 min on day 10. Although the correlation between the signal at a fixed time point and the peak signal was high, the signal at 5 or 10 min normalized for the peak signal was lower for earlier days, which caused overestimation of tumor growth. The time course of the signals after D-luciferin injection may vary with time after cell inoculation, and this variation should be considered when determining the imaging protocol for quantitative bioluminescence tumor monitoring. Hindawi Publishing Corporation 2010 2010-07-05 /pmc/articles/PMC2910476/ /pubmed/20671955 http://dx.doi.org/10.1155/2010/471408 Text en Copyright © 2010 Yusuke Inoue et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Inoue, Yusuke
Kiryu, Shigeru
Watanabe, Makoto
Tojo, Arinobu
Ohtomo, Kuni
Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging
title Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging
title_full Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging
title_fullStr Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging
title_full_unstemmed Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging
title_short Timing of Imaging after D-Luciferin Injection Affects the Longitudinal Assessment of Tumor Growth Using In Vivo Bioluminescence Imaging
title_sort timing of imaging after d-luciferin injection affects the longitudinal assessment of tumor growth using in vivo bioluminescence imaging
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910476/
https://www.ncbi.nlm.nih.gov/pubmed/20671955
http://dx.doi.org/10.1155/2010/471408
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