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Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction
Numerous F-actin containing structures are involved in regulating protrusion of membrane at the leading edge of motile cells. We have investigated the structure and dynamics of filopodia as they relate to events at the leading edge and the function of the trailing actin networks. We have found that...
Autores principales: | , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910478/ https://www.ncbi.nlm.nih.gov/pubmed/20671957 http://dx.doi.org/10.1155/2010/507821 |
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author | Xue, Fei Janzen, Deanna M. Knecht, David A. |
author_facet | Xue, Fei Janzen, Deanna M. Knecht, David A. |
author_sort | Xue, Fei |
collection | PubMed |
description | Numerous F-actin containing structures are involved in regulating protrusion of membrane at the leading edge of motile cells. We have investigated the structure and dynamics of filopodia as they relate to events at the leading edge and the function of the trailing actin networks. We have found that although filopodia contain parallel bundles of actin, they contain a surprisingly nonuniform spatial and temporal distribution of actin binding proteins. Along the length of the actin filaments in a single filopodium, the most distal portion contains primarily T-plastin, while the proximal portion is primarily bound by α-actinin and coronin. Some filopodia are stationary, but lateral filopodia move with respect to the leading edge. They appear to form a mechanical link between the actin polymerization network at the front of the cell and the myosin motor activity in the cell body. The direction of lateral filopodial movement is associated with the direction of cell migration. When lateral filopodia initiate from and move toward only one side of a cell, the cell will turn opposite to the direction of filopodial flow. Therefore, this filopodia-myosin II system allows actin polymerization driven protrusion forces and myosin II mediated contractile force to be mechanically coordinated. |
format | Text |
id | pubmed-2910478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-29104782010-07-29 Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction Xue, Fei Janzen, Deanna M. Knecht, David A. Int J Cell Biol Research Article Numerous F-actin containing structures are involved in regulating protrusion of membrane at the leading edge of motile cells. We have investigated the structure and dynamics of filopodia as they relate to events at the leading edge and the function of the trailing actin networks. We have found that although filopodia contain parallel bundles of actin, they contain a surprisingly nonuniform spatial and temporal distribution of actin binding proteins. Along the length of the actin filaments in a single filopodium, the most distal portion contains primarily T-plastin, while the proximal portion is primarily bound by α-actinin and coronin. Some filopodia are stationary, but lateral filopodia move with respect to the leading edge. They appear to form a mechanical link between the actin polymerization network at the front of the cell and the myosin motor activity in the cell body. The direction of lateral filopodial movement is associated with the direction of cell migration. When lateral filopodia initiate from and move toward only one side of a cell, the cell will turn opposite to the direction of filopodial flow. Therefore, this filopodia-myosin II system allows actin polymerization driven protrusion forces and myosin II mediated contractile force to be mechanically coordinated. Hindawi Publishing Corporation 2010 2010-07-06 /pmc/articles/PMC2910478/ /pubmed/20671957 http://dx.doi.org/10.1155/2010/507821 Text en Copyright © 2010 Fei Xue et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xue, Fei Janzen, Deanna M. Knecht, David A. Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction |
title | Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction |
title_full | Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction |
title_fullStr | Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction |
title_full_unstemmed | Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction |
title_short | Contribution of Filopodia to Cell Migration: A Mechanical Link between Protrusion and Contraction |
title_sort | contribution of filopodia to cell migration: a mechanical link between protrusion and contraction |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910478/ https://www.ncbi.nlm.nih.gov/pubmed/20671957 http://dx.doi.org/10.1155/2010/507821 |
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