Cargando…
Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages
Here, we studied in vitro cytokine production by splenic macrophages obtained from young and aged BALB/c wild type (WT) and IL-6 knockout (IL-6 KO) mice. Relative to macrophages obtained from young WT mice given lipopolysaccharide (LPS), those from aged WT mice had decreased production of proinflamm...
Autores principales: | , , , , , , |
---|---|
Formato: | Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2010
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910493/ https://www.ncbi.nlm.nih.gov/pubmed/20671912 http://dx.doi.org/10.1155/2010/475139 |
_version_ | 1782184393205350400 |
---|---|
author | Gomez, Christian R. Karavitis, John Palmer, Jessica L. Faunce, Douglas E. Ramirez, Luis Nomellini, Vanessa Kovacs, Elizabeth J. |
author_facet | Gomez, Christian R. Karavitis, John Palmer, Jessica L. Faunce, Douglas E. Ramirez, Luis Nomellini, Vanessa Kovacs, Elizabeth J. |
author_sort | Gomez, Christian R. |
collection | PubMed |
description | Here, we studied in vitro cytokine production by splenic macrophages obtained from young and aged BALB/c wild type (WT) and IL-6 knockout (IL-6 KO) mice. Relative to macrophages obtained from young WT mice given lipopolysaccharide (LPS), those from aged WT mice had decreased production of proinflammatory cytokines. In contrast, when compared to macrophages from young IL-6 KO mice, LPS stimulation yielded higher levels of these cytokines by cells from aged IL-6 KO mice. Aging or IL-6 deficiency did not affected the percentage of F4/80(+) macrophages, or the surface expression of Toll-like receptor 4 (TLR4) and components of the IL-6 receptor. Overall, our results indicate that IL-6 plays a role in regulating the age-related defects in macrophages through alteration of proinflammatory cytokines, adding to the complexity of IL-6-mediated impairment of immune cell function with increasing age. |
format | Text |
id | pubmed-2910493 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-29104932010-07-29 Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages Gomez, Christian R. Karavitis, John Palmer, Jessica L. Faunce, Douglas E. Ramirez, Luis Nomellini, Vanessa Kovacs, Elizabeth J. Mediators Inflamm Research Article Here, we studied in vitro cytokine production by splenic macrophages obtained from young and aged BALB/c wild type (WT) and IL-6 knockout (IL-6 KO) mice. Relative to macrophages obtained from young WT mice given lipopolysaccharide (LPS), those from aged WT mice had decreased production of proinflammatory cytokines. In contrast, when compared to macrophages from young IL-6 KO mice, LPS stimulation yielded higher levels of these cytokines by cells from aged IL-6 KO mice. Aging or IL-6 deficiency did not affected the percentage of F4/80(+) macrophages, or the surface expression of Toll-like receptor 4 (TLR4) and components of the IL-6 receptor. Overall, our results indicate that IL-6 plays a role in regulating the age-related defects in macrophages through alteration of proinflammatory cytokines, adding to the complexity of IL-6-mediated impairment of immune cell function with increasing age. Hindawi Publishing Corporation 2010 2010-07-07 /pmc/articles/PMC2910493/ /pubmed/20671912 http://dx.doi.org/10.1155/2010/475139 Text en Copyright © 2010 Christian R. Gomez et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Gomez, Christian R. Karavitis, John Palmer, Jessica L. Faunce, Douglas E. Ramirez, Luis Nomellini, Vanessa Kovacs, Elizabeth J. Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
title | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
title_full | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
title_fullStr | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
title_full_unstemmed | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
title_short | Interleukin-6 Contributes to Age-Related Alteration of Cytokine Production by Macrophages |
title_sort | interleukin-6 contributes to age-related alteration of cytokine production by macrophages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910493/ https://www.ncbi.nlm.nih.gov/pubmed/20671912 http://dx.doi.org/10.1155/2010/475139 |
work_keys_str_mv | AT gomezchristianr interleukin6contributestoagerelatedalterationofcytokineproductionbymacrophages AT karavitisjohn interleukin6contributestoagerelatedalterationofcytokineproductionbymacrophages AT palmerjessical interleukin6contributestoagerelatedalterationofcytokineproductionbymacrophages AT fauncedouglase interleukin6contributestoagerelatedalterationofcytokineproductionbymacrophages AT ramirezluis interleukin6contributestoagerelatedalterationofcytokineproductionbymacrophages AT nomellinivanessa interleukin6contributestoagerelatedalterationofcytokineproductionbymacrophages AT kovacselizabethj interleukin6contributestoagerelatedalterationofcytokineproductionbymacrophages |