Global fitness profiling of fission yeast deletion strains by barcode sequencing

A genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeast Schizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative mea...

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Detalles Bibliográficos
Autores principales: Han, Tian Xu, Xu, Xing-Ya, Zhang, Mei-Jun, Peng, Xu, Du, Li-Lin
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911108/
https://www.ncbi.nlm.nih.gov/pubmed/20537132
http://dx.doi.org/10.1186/gb-2010-11-6-r60
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author Han, Tian Xu
Xu, Xing-Ya
Zhang, Mei-Jun
Peng, Xu
Du, Li-Lin
author_facet Han, Tian Xu
Xu, Xing-Ya
Zhang, Mei-Jun
Peng, Xu
Du, Li-Lin
author_sort Han, Tian Xu
collection PubMed
description A genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeast Schizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative measurement of the fitness of fission yeast deletion strains by barcode sequencing.
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spelling pubmed-29111082010-07-28 Global fitness profiling of fission yeast deletion strains by barcode sequencing Han, Tian Xu Xu, Xing-Ya Zhang, Mei-Jun Peng, Xu Du, Li-Lin Genome Biol Method A genome-wide deletion library is a powerful tool for probing gene functions and one has recently become available for the fission yeast Schizosaccharomyces pombe. Here we use deep sequencing to accurately characterize the barcode sequences in the deletion library, thus enabling the quantitative measurement of the fitness of fission yeast deletion strains by barcode sequencing. BioMed Central 2010 2010-06-10 /pmc/articles/PMC2911108/ /pubmed/20537132 http://dx.doi.org/10.1186/gb-2010-11-6-r60 Text en Copyright ©2010 Han et al.; licensee BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Han, Tian Xu
Xu, Xing-Ya
Zhang, Mei-Jun
Peng, Xu
Du, Li-Lin
Global fitness profiling of fission yeast deletion strains by barcode sequencing
title Global fitness profiling of fission yeast deletion strains by barcode sequencing
title_full Global fitness profiling of fission yeast deletion strains by barcode sequencing
title_fullStr Global fitness profiling of fission yeast deletion strains by barcode sequencing
title_full_unstemmed Global fitness profiling of fission yeast deletion strains by barcode sequencing
title_short Global fitness profiling of fission yeast deletion strains by barcode sequencing
title_sort global fitness profiling of fission yeast deletion strains by barcode sequencing
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911108/
https://www.ncbi.nlm.nih.gov/pubmed/20537132
http://dx.doi.org/10.1186/gb-2010-11-6-r60
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AT zhangmeijun globalfitnessprofilingoffissionyeastdeletionstrainsbybarcodesequencing
AT pengxu globalfitnessprofilingoffissionyeastdeletionstrainsbybarcodesequencing
AT dulilin globalfitnessprofilingoffissionyeastdeletionstrainsbybarcodesequencing

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