Whole exome capture in solution with 3 Gbp of data

We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows the discovery of greater than 95% of all expected heterozygous singe base variants, requires as little as 3 Gbp of raw sequence data and constitutes...

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Detalles Bibliográficos
Autores principales: Bainbridge, Matthew N, Wang, Min, Burgess, Daniel L, Kovar, Christie, Rodesch, Matthew J, D'Ascenzo, Mark, Kitzman, Jacob, Wu, Yuan-Qing, Newsham, Irene, Richmond, Todd A, Jeddeloh, Jeffrey A, Muzny, Donna, Albert, Thomas J, Gibbs, Richard A
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Method
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911110/
https://www.ncbi.nlm.nih.gov/pubmed/20565776
http://dx.doi.org/10.1186/gb-2010-11-6-r62
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author Bainbridge, Matthew N
Wang, Min
Burgess, Daniel L
Kovar, Christie
Rodesch, Matthew J
D'Ascenzo, Mark
Kitzman, Jacob
Wu, Yuan-Qing
Newsham, Irene
Richmond, Todd A
Jeddeloh, Jeffrey A
Muzny, Donna
Albert, Thomas J
Gibbs, Richard A
author_facet Bainbridge, Matthew N
Wang, Min
Burgess, Daniel L
Kovar, Christie
Rodesch, Matthew J
D'Ascenzo, Mark
Kitzman, Jacob
Wu, Yuan-Qing
Newsham, Irene
Richmond, Todd A
Jeddeloh, Jeffrey A
Muzny, Donna
Albert, Thomas J
Gibbs, Richard A
author_sort Bainbridge, Matthew N
collection PubMed
description We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows the discovery of greater than 95% of all expected heterozygous singe base variants, requires as little as 3 Gbp of raw sequence data and constitutes an effective tool for identifying rare coding alleles in large scale genomic studies.
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spelling pubmed-29111102010-07-28 Whole exome capture in solution with 3 Gbp of data Bainbridge, Matthew N Wang, Min Burgess, Daniel L Kovar, Christie Rodesch, Matthew J D'Ascenzo, Mark Kitzman, Jacob Wu, Yuan-Qing Newsham, Irene Richmond, Todd A Jeddeloh, Jeffrey A Muzny, Donna Albert, Thomas J Gibbs, Richard A Genome Biol Method We have developed a solution-based method for targeted DNA capture-sequencing that is directed to the complete human exome. Using this approach allows the discovery of greater than 95% of all expected heterozygous singe base variants, requires as little as 3 Gbp of raw sequence data and constitutes an effective tool for identifying rare coding alleles in large scale genomic studies. BioMed Central 2010 2010-06-17 /pmc/articles/PMC2911110/ /pubmed/20565776 http://dx.doi.org/10.1186/gb-2010-11-6-r62 Text en Copyright ©2010 Bainbridge et al.; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Method
Bainbridge, Matthew N
Wang, Min
Burgess, Daniel L
Kovar, Christie
Rodesch, Matthew J
D'Ascenzo, Mark
Kitzman, Jacob
Wu, Yuan-Qing
Newsham, Irene
Richmond, Todd A
Jeddeloh, Jeffrey A
Muzny, Donna
Albert, Thomas J
Gibbs, Richard A
Whole exome capture in solution with 3 Gbp of data
title Whole exome capture in solution with 3 Gbp of data
title_full Whole exome capture in solution with 3 Gbp of data
title_fullStr Whole exome capture in solution with 3 Gbp of data
title_full_unstemmed Whole exome capture in solution with 3 Gbp of data
title_short Whole exome capture in solution with 3 Gbp of data
title_sort whole exome capture in solution with 3 gbp of data
topic Method
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2911110/
https://www.ncbi.nlm.nih.gov/pubmed/20565776
http://dx.doi.org/10.1186/gb-2010-11-6-r62
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