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Biogenesis of the Inner Membrane Complex Is Dependent on Vesicular Transport by the Alveolate Specific GTPase Rab11B

Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo w...

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Autores principales: Agop-Nersesian, Carolina, Egarter, Saskia, Langsley, Gordon, Foth, Bernardo J., Ferguson, David J. P., Meissner, Markus
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912401/
https://www.ncbi.nlm.nih.gov/pubmed/20686666
http://dx.doi.org/10.1371/journal.ppat.1001029
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author Agop-Nersesian, Carolina
Egarter, Saskia
Langsley, Gordon
Foth, Bernardo J.
Ferguson, David J. P.
Meissner, Markus
author_facet Agop-Nersesian, Carolina
Egarter, Saskia
Langsley, Gordon
Foth, Bernardo J.
Ferguson, David J. P.
Meissner, Markus
author_sort Agop-Nersesian, Carolina
collection PubMed
description Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo within the mother cell in a process described as internal budding. We hypothesized that an alveolate specific factor is involved in the specific transport of vesicles from the Golgi to the IMC and identified the small GTPase Rab11B as an alveolate specific Rab-GTPase that localises to the growing end of the IMC during replication of Toxoplasma gondii. Conditional interference with Rab11B function leads to a profound defect in IMC biogenesis, indicating that Rab11B is required for the transport of Golgi derived vesicles to the nascent IMC of the daughter cell. Curiously, a block in IMC biogenesis did not affect formation of sub-pellicular microtubules, indicating that IMC biogenesis and formation of sub-pellicular microtubules is not mechanistically linked. We propose a model where Rab11B specifically transports vesicles derived from the Golgi to the immature IMC of the growing daughter parasites.
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spelling pubmed-29124012010-08-03 Biogenesis of the Inner Membrane Complex Is Dependent on Vesicular Transport by the Alveolate Specific GTPase Rab11B Agop-Nersesian, Carolina Egarter, Saskia Langsley, Gordon Foth, Bernardo J. Ferguson, David J. P. Meissner, Markus PLoS Pathog Research Article Apicomplexan parasites belong to a recently recognised group of protozoa referred to as Alveolata. These protists contain membranous sacs (alveoli) beneath the plasma membrane, termed the Inner Membrane Complex (IMC) in the case of Apicomplexa. During parasite replication the IMC is formed de novo within the mother cell in a process described as internal budding. We hypothesized that an alveolate specific factor is involved in the specific transport of vesicles from the Golgi to the IMC and identified the small GTPase Rab11B as an alveolate specific Rab-GTPase that localises to the growing end of the IMC during replication of Toxoplasma gondii. Conditional interference with Rab11B function leads to a profound defect in IMC biogenesis, indicating that Rab11B is required for the transport of Golgi derived vesicles to the nascent IMC of the daughter cell. Curiously, a block in IMC biogenesis did not affect formation of sub-pellicular microtubules, indicating that IMC biogenesis and formation of sub-pellicular microtubules is not mechanistically linked. We propose a model where Rab11B specifically transports vesicles derived from the Golgi to the immature IMC of the growing daughter parasites. Public Library of Science 2010-07-29 /pmc/articles/PMC2912401/ /pubmed/20686666 http://dx.doi.org/10.1371/journal.ppat.1001029 Text en Agop-Nersesian et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Agop-Nersesian, Carolina
Egarter, Saskia
Langsley, Gordon
Foth, Bernardo J.
Ferguson, David J. P.
Meissner, Markus
Biogenesis of the Inner Membrane Complex Is Dependent on Vesicular Transport by the Alveolate Specific GTPase Rab11B
title Biogenesis of the Inner Membrane Complex Is Dependent on Vesicular Transport by the Alveolate Specific GTPase Rab11B
title_full Biogenesis of the Inner Membrane Complex Is Dependent on Vesicular Transport by the Alveolate Specific GTPase Rab11B
title_fullStr Biogenesis of the Inner Membrane Complex Is Dependent on Vesicular Transport by the Alveolate Specific GTPase Rab11B
title_full_unstemmed Biogenesis of the Inner Membrane Complex Is Dependent on Vesicular Transport by the Alveolate Specific GTPase Rab11B
title_short Biogenesis of the Inner Membrane Complex Is Dependent on Vesicular Transport by the Alveolate Specific GTPase Rab11B
title_sort biogenesis of the inner membrane complex is dependent on vesicular transport by the alveolate specific gtpase rab11b
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912401/
https://www.ncbi.nlm.nih.gov/pubmed/20686666
http://dx.doi.org/10.1371/journal.ppat.1001029
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