Cargando…

Peripheral blood T Regulatory cell counts may not predict transplant rejection

BACKGROUND: Recent evidence shows that allograft survival rates show a positive correlation with the number of circulating T regulatory cells (Tregs). This study investigated both the number and the cytokine profiles exhibited by Foxp3(+ )Tregs in blood, spleen and lymph nodes of Lewis rat recipient...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Yuchuan, Shan, Juan, Zhang, Chuntao, Zhang, Jie, Feng, Li, Li, Shengfu, Li, Youping
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912834/
https://www.ncbi.nlm.nih.gov/pubmed/20633262
http://dx.doi.org/10.1186/1471-2172-11-40
_version_ 1782184631768973312
author Huang, Yuchuan
Shan, Juan
Zhang, Chuntao
Zhang, Jie
Feng, Li
Li, Shengfu
Li, Youping
author_facet Huang, Yuchuan
Shan, Juan
Zhang, Chuntao
Zhang, Jie
Feng, Li
Li, Shengfu
Li, Youping
author_sort Huang, Yuchuan
collection PubMed
description BACKGROUND: Recent evidence shows that allograft survival rates show a positive correlation with the number of circulating T regulatory cells (Tregs). This study investigated both the number and the cytokine profiles exhibited by Foxp3(+ )Tregs in blood, spleen and lymph nodes of Lewis rat recipients of BN rat cardiac allografts after a single-dose of Rapamycin (RAPA). RESULTS: Rats were divided into three groups: control group (containing healthy control and acute rejection group), and recipients treated with a single dose of RAPA on either Day 1 (1D group)or Day 3 (3D group) post-transplant. We analyzed the number of Foxp3+Tregs and the expression of Foxp3 and cytokines in the peripheral blood and the peripheral lymphoid tissues. No difference was found in the numbers of circulating Foxp3+ Tregs between these three groups. RAPA administration significantly increased Foxp3 expression in peripheral lymphoid tissues after a single dose of RAPA on Day 3 post-transplant. Foxp3+Tregs inhibited the activity of effector T cells (T(eff)) via the secretion of TGF-β1. CONCLUSION: The number of Tregs in the recipient's blood may not be a good predictor of transplant rejection. Foxp3+Tregs inhibit the activity of T(eff )cells mainly in the peripheral lymphoid tissues.
format Text
id pubmed-2912834
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-29128342010-07-31 Peripheral blood T Regulatory cell counts may not predict transplant rejection Huang, Yuchuan Shan, Juan Zhang, Chuntao Zhang, Jie Feng, Li Li, Shengfu Li, Youping BMC Immunol Research Article BACKGROUND: Recent evidence shows that allograft survival rates show a positive correlation with the number of circulating T regulatory cells (Tregs). This study investigated both the number and the cytokine profiles exhibited by Foxp3(+ )Tregs in blood, spleen and lymph nodes of Lewis rat recipients of BN rat cardiac allografts after a single-dose of Rapamycin (RAPA). RESULTS: Rats were divided into three groups: control group (containing healthy control and acute rejection group), and recipients treated with a single dose of RAPA on either Day 1 (1D group)or Day 3 (3D group) post-transplant. We analyzed the number of Foxp3+Tregs and the expression of Foxp3 and cytokines in the peripheral blood and the peripheral lymphoid tissues. No difference was found in the numbers of circulating Foxp3+ Tregs between these three groups. RAPA administration significantly increased Foxp3 expression in peripheral lymphoid tissues after a single dose of RAPA on Day 3 post-transplant. Foxp3+Tregs inhibited the activity of effector T cells (T(eff)) via the secretion of TGF-β1. CONCLUSION: The number of Tregs in the recipient's blood may not be a good predictor of transplant rejection. Foxp3+Tregs inhibit the activity of T(eff )cells mainly in the peripheral lymphoid tissues. BioMed Central 2010-07-15 /pmc/articles/PMC2912834/ /pubmed/20633262 http://dx.doi.org/10.1186/1471-2172-11-40 Text en Copyright ©2010 Huang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Huang, Yuchuan
Shan, Juan
Zhang, Chuntao
Zhang, Jie
Feng, Li
Li, Shengfu
Li, Youping
Peripheral blood T Regulatory cell counts may not predict transplant rejection
title Peripheral blood T Regulatory cell counts may not predict transplant rejection
title_full Peripheral blood T Regulatory cell counts may not predict transplant rejection
title_fullStr Peripheral blood T Regulatory cell counts may not predict transplant rejection
title_full_unstemmed Peripheral blood T Regulatory cell counts may not predict transplant rejection
title_short Peripheral blood T Regulatory cell counts may not predict transplant rejection
title_sort peripheral blood t regulatory cell counts may not predict transplant rejection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912834/
https://www.ncbi.nlm.nih.gov/pubmed/20633262
http://dx.doi.org/10.1186/1471-2172-11-40
work_keys_str_mv AT huangyuchuan peripheralbloodtregulatorycellcountsmaynotpredicttransplantrejection
AT shanjuan peripheralbloodtregulatorycellcountsmaynotpredicttransplantrejection
AT zhangchuntao peripheralbloodtregulatorycellcountsmaynotpredicttransplantrejection
AT zhangjie peripheralbloodtregulatorycellcountsmaynotpredicttransplantrejection
AT fengli peripheralbloodtregulatorycellcountsmaynotpredicttransplantrejection
AT lishengfu peripheralbloodtregulatorycellcountsmaynotpredicttransplantrejection
AT liyouping peripheralbloodtregulatorycellcountsmaynotpredicttransplantrejection