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Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices

Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies had documented concurrent impairment in neutrophil chemotaxis with these post...

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Autores principales: Butler, Kathryn L., Ambravaneswaran, Vijayakrishnan, Agrawal, Nitin, Bilodeau, Maryelizabeth, Toner, Mehmet, Tompkins, Ronald G., Fagan, Shawn, Irimia, Daniel
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912851/
https://www.ncbi.nlm.nih.gov/pubmed/20689600
http://dx.doi.org/10.1371/journal.pone.0011921
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author Butler, Kathryn L.
Ambravaneswaran, Vijayakrishnan
Agrawal, Nitin
Bilodeau, Maryelizabeth
Toner, Mehmet
Tompkins, Ronald G.
Fagan, Shawn
Irimia, Daniel
author_facet Butler, Kathryn L.
Ambravaneswaran, Vijayakrishnan
Agrawal, Nitin
Bilodeau, Maryelizabeth
Toner, Mehmet
Tompkins, Ronald G.
Fagan, Shawn
Irimia, Daniel
author_sort Butler, Kathryn L.
collection PubMed
description Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies had documented concurrent impairment in neutrophil chemotaxis with these post-burn inflammatory changes. While a few studies suggest that a link between neutrophil motility and patient mortality might exist, so far, cumbersome assays have prohibited exploration of the prognostic and diagnostic significance of chemotaxis after burn injury. To address this need, we developed a microfluidic device that is simple to operate and allows for precise and robust measurements of chemotaxis speed and persistence characteristics at single-cell resolution. Using this assay, we established a reference set of migration speed values for neutrophils from healthy subjects. Comparisons with samples from burn patients revealed impaired directional migration speed starting as early as 24 hours after burn injury, reaching a minimum at 72–120 hours, correlated to the size of the burn injury and potentially serving as an early indicator for concurrent infections. Further characterization of neutrophil chemotaxis using this new assay may have important diagnostic implications not only for burn patients but also for patients afflicted by other diseases that compromise neutrophil functions.
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spelling pubmed-29128512010-08-04 Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices Butler, Kathryn L. Ambravaneswaran, Vijayakrishnan Agrawal, Nitin Bilodeau, Maryelizabeth Toner, Mehmet Tompkins, Ronald G. Fagan, Shawn Irimia, Daniel PLoS One Research Article Thermal injury triggers a fulminant inflammatory cascade that heralds shock, end-organ failure, and ultimately sepsis and death. Emerging evidence points to a critical role for the innate immune system, and several studies had documented concurrent impairment in neutrophil chemotaxis with these post-burn inflammatory changes. While a few studies suggest that a link between neutrophil motility and patient mortality might exist, so far, cumbersome assays have prohibited exploration of the prognostic and diagnostic significance of chemotaxis after burn injury. To address this need, we developed a microfluidic device that is simple to operate and allows for precise and robust measurements of chemotaxis speed and persistence characteristics at single-cell resolution. Using this assay, we established a reference set of migration speed values for neutrophils from healthy subjects. Comparisons with samples from burn patients revealed impaired directional migration speed starting as early as 24 hours after burn injury, reaching a minimum at 72–120 hours, correlated to the size of the burn injury and potentially serving as an early indicator for concurrent infections. Further characterization of neutrophil chemotaxis using this new assay may have important diagnostic implications not only for burn patients but also for patients afflicted by other diseases that compromise neutrophil functions. Public Library of Science 2010-07-30 /pmc/articles/PMC2912851/ /pubmed/20689600 http://dx.doi.org/10.1371/journal.pone.0011921 Text en Butler et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Butler, Kathryn L.
Ambravaneswaran, Vijayakrishnan
Agrawal, Nitin
Bilodeau, Maryelizabeth
Toner, Mehmet
Tompkins, Ronald G.
Fagan, Shawn
Irimia, Daniel
Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices
title Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices
title_full Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices
title_fullStr Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices
title_full_unstemmed Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices
title_short Burn Injury Reduces Neutrophil Directional Migration Speed in Microfluidic Devices
title_sort burn injury reduces neutrophil directional migration speed in microfluidic devices
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2912851/
https://www.ncbi.nlm.nih.gov/pubmed/20689600
http://dx.doi.org/10.1371/journal.pone.0011921
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