New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA

Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. h...

Descripción completa

Detalles Bibliográficos
Autores principales: Pascual, Andres, Jaton, Katia, Ninet, Béatrice, Bille, Jacques, Greub, Gilbert
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913506/
https://www.ncbi.nlm.nih.gov/pubmed/20706532
http://dx.doi.org/10.1155/2010/317512
_version_ 1782184671708184576
author Pascual, Andres
Jaton, Katia
Ninet, Béatrice
Bille, Jacques
Greub, Gilbert
author_facet Pascual, Andres
Jaton, Katia
Ninet, Béatrice
Bille, Jacques
Greub, Gilbert
author_sort Pascual, Andres
collection PubMed
description Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. hominis in three patients with severe extragenital infections.
format Text
id pubmed-2913506
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Hindawi Publishing Corporation
record_format MEDLINE/PubMed
spelling pubmed-29135062010-08-12 New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA Pascual, Andres Jaton, Katia Ninet, Béatrice Bille, Jacques Greub, Gilbert Int J Microbiol Research Article Mycoplasma hominis is a fastidious micro-organism causing genital and extragenital infections. We developed a specific real-time PCR that exhibits high sensitivity and low intrarun and interrun variabilities. When applied to clinical samples, this quantitative PCR allowed to confirm the role of M. hominis in three patients with severe extragenital infections. Hindawi Publishing Corporation 2010 2010-07-25 /pmc/articles/PMC2913506/ /pubmed/20706532 http://dx.doi.org/10.1155/2010/317512 Text en Copyright © 2010 Andres Pascual et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Pascual, Andres
Jaton, Katia
Ninet, Béatrice
Bille, Jacques
Greub, Gilbert
New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA
title New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA
title_full New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA
title_fullStr New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA
title_full_unstemmed New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA
title_short New Diagnostic Real-Time PCR for Specific Detection of Mycoplasma hominis DNA
title_sort new diagnostic real-time pcr for specific detection of mycoplasma hominis dna
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913506/
https://www.ncbi.nlm.nih.gov/pubmed/20706532
http://dx.doi.org/10.1155/2010/317512
work_keys_str_mv AT pascualandres newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna
AT jatonkatia newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna
AT ninetbeatrice newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna
AT billejacques newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna
AT greubgilbert newdiagnosticrealtimepcrforspecificdetectionofmycoplasmahominisdna

Ejemplares similares