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Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites
The aim of this study was to assess the genotoxic effects of environmental chemicals on residents living near landfills. The study was based on samples of amniotic fluid from women living in the intensely polluted areas around the Campania region of Italy compared to a nonexposed control group. We e...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913803/ https://www.ncbi.nlm.nih.gov/pubmed/20706694 http://dx.doi.org/10.1155/2010/251767 |
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author | Guida, Maurizio Guida, Marco De Felice, Bruna Santafede, Daniela D'Alessandro, Raffaella Di Spiezio Sardo, Attilio Scognamiglio, Marianna Ferrara, Cinzia Bifulco, Giuseppe Nappi, Carmine |
author_facet | Guida, Maurizio Guida, Marco De Felice, Bruna Santafede, Daniela D'Alessandro, Raffaella Di Spiezio Sardo, Attilio Scognamiglio, Marianna Ferrara, Cinzia Bifulco, Giuseppe Nappi, Carmine |
author_sort | Guida, Maurizio |
collection | PubMed |
description | The aim of this study was to assess the genotoxic effects of environmental chemicals on residents living near landfills. The study was based on samples of amniotic fluid from women living in the intensely polluted areas around the Campania region of Italy compared to a nonexposed control group. We evaluated the genetic effects that this amniotic fluids collected in contaminated sites had on Paracentrotus lividus embryos. DNA damage was detected through changes in RAPD (Random Amplified Polymorphism DNA) profiles. The absence of the amplified DNA fragments indicated deletions in Paracentrotus lividus DNA exposed to the contaminated amniotic fluids when compared to equal exposure to uncontaminated fluids. These results show the ability of RAPD-PCR to detect and isolate DNA sequences representing genetic alterations induced in P. lividus embryos. Using this method, we identified two candidate target regions for DNA alterations in the genome of P. lividus. Our research indicates that RAPD-PCR in P. lividus embryo DNA can provide a molecular approach for studying DNA damage from pollutants that can impact human health. To our knowledge, this is the first time that assessment of DNA damage in P. lividus embryos has been tested using the RAPD strategy after exposure to amniotic fluid from residents near waste landfill sites. |
format | Text |
id | pubmed-2913803 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-29138032010-08-12 Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites Guida, Maurizio Guida, Marco De Felice, Bruna Santafede, Daniela D'Alessandro, Raffaella Di Spiezio Sardo, Attilio Scognamiglio, Marianna Ferrara, Cinzia Bifulco, Giuseppe Nappi, Carmine J Biomed Biotechnol Research Article The aim of this study was to assess the genotoxic effects of environmental chemicals on residents living near landfills. The study was based on samples of amniotic fluid from women living in the intensely polluted areas around the Campania region of Italy compared to a nonexposed control group. We evaluated the genetic effects that this amniotic fluids collected in contaminated sites had on Paracentrotus lividus embryos. DNA damage was detected through changes in RAPD (Random Amplified Polymorphism DNA) profiles. The absence of the amplified DNA fragments indicated deletions in Paracentrotus lividus DNA exposed to the contaminated amniotic fluids when compared to equal exposure to uncontaminated fluids. These results show the ability of RAPD-PCR to detect and isolate DNA sequences representing genetic alterations induced in P. lividus embryos. Using this method, we identified two candidate target regions for DNA alterations in the genome of P. lividus. Our research indicates that RAPD-PCR in P. lividus embryo DNA can provide a molecular approach for studying DNA damage from pollutants that can impact human health. To our knowledge, this is the first time that assessment of DNA damage in P. lividus embryos has been tested using the RAPD strategy after exposure to amniotic fluid from residents near waste landfill sites. Hindawi Publishing Corporation 2010 2010-07-11 /pmc/articles/PMC2913803/ /pubmed/20706694 http://dx.doi.org/10.1155/2010/251767 Text en Copyright © 2010 Maurizio Guida et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Guida, Maurizio Guida, Marco De Felice, Bruna Santafede, Daniela D'Alessandro, Raffaella Di Spiezio Sardo, Attilio Scognamiglio, Marianna Ferrara, Cinzia Bifulco, Giuseppe Nappi, Carmine Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites |
title | Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites |
title_full | Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites |
title_fullStr | Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites |
title_full_unstemmed | Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites |
title_short | Assessment of DNA Damage by RAPD in Paracentrotus lividus Embryos Exposed to Amniotic Fluid from Residents Living Close to Waste Landfill Sites |
title_sort | assessment of dna damage by rapd in paracentrotus lividus embryos exposed to amniotic fluid from residents living close to waste landfill sites |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913803/ https://www.ncbi.nlm.nih.gov/pubmed/20706694 http://dx.doi.org/10.1155/2010/251767 |
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