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Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188

In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or p...

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Detalles Bibliográficos
Autores principales: Tsoneva, Iana, Iordanov, Iordan, Berger, Annette J., Tomov, Toma, Nikolova, Biliana, Mudrov, Nikola, Berger, Martin R.
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913842/
https://www.ncbi.nlm.nih.gov/pubmed/20706647
http://dx.doi.org/10.1155/2010/314213
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author Tsoneva, Iana
Iordanov, Iordan
Berger, Annette J.
Tomov, Toma
Nikolova, Biliana
Mudrov, Nikola
Berger, Martin R.
author_facet Tsoneva, Iana
Iordanov, Iordan
Berger, Annette J.
Tomov, Toma
Nikolova, Biliana
Mudrov, Nikola
Berger, Martin R.
author_sort Tsoneva, Iana
collection PubMed
description In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors.
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spelling pubmed-29138422010-08-12 Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188 Tsoneva, Iana Iordanov, Iordan Berger, Annette J. Tomov, Toma Nikolova, Biliana Mudrov, Nikola Berger, Martin R. J Biomed Biotechnol Research Article In the present study it is shown that poloxamer 188, added before or immediately after an electrical pulse used for electroporation, decreases the number of dead cells and at the same time does not reduce the number of reversible electropores through which small molecules (cisplatin, bleomycin, or propidium iodide) can pass/diffuse. It was suggested that hydrophobic sections of poloxamer 188 molecules are incorporated into the edges of pores and that their hydrophilic parts act as brushy pore structures. The formation of brushy pores may reduce the expansion of pores and delay the irreversible electropermeability. Tumors were implanted subcutaneously in both flanks of nude mice using HeLa cells, transfected with genes for red fluorescent protein and luciferase. The volume of tumors stopped to grow after electrochemotherapy and the use of poloxamer 188 reduced the edema near the electrode and around the subcutaneously growing tumors. Hindawi Publishing Corporation 2010 2010-07-25 /pmc/articles/PMC2913842/ /pubmed/20706647 http://dx.doi.org/10.1155/2010/314213 Text en Copyright © 2010 Iana Tsoneva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Tsoneva, Iana
Iordanov, Iordan
Berger, Annette J.
Tomov, Toma
Nikolova, Biliana
Mudrov, Nikola
Berger, Martin R.
Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188
title Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188
title_full Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188
title_fullStr Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188
title_full_unstemmed Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188
title_short Electrodelivery of Drugs into Cancer Cells in the Presence of Poloxamer 188
title_sort electrodelivery of drugs into cancer cells in the presence of poloxamer 188
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913842/
https://www.ncbi.nlm.nih.gov/pubmed/20706647
http://dx.doi.org/10.1155/2010/314213
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