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Novel Method of Cell-Free In Vitro Synthesis of the Human Fibroblast Growth Factor 1 Gene
Recombinant DNA projects generally involve cell-based gene cloning. However, because template DNA is not always readily available, in vitro chemical synthesis of complete genes from DNA oligonucleotides is becoming the preferred method for cloning. This article describes a new, rapid procedure based...
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913909/ https://www.ncbi.nlm.nih.gov/pubmed/20706664 http://dx.doi.org/10.1155/2010/971340 |