Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein

HSV-1 viral capsid maturation and egress from the nucleus constitutes a self-controlled process of interactions between host cytoplasmic membrane proteins and viral capsid proteins. In this study, a member of the tetraspanin superfamily, CTMP-7, was shown to physically interact with HSV-1 protein VP...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Lei, Liu, Longding, Che, Yanchun, Wang, Lichun, Jiang, Li, Dong, Chenghong, Zhang, Ying, Li, Qihan
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913958/
https://www.ncbi.nlm.nih.gov/pubmed/20630051
http://dx.doi.org/10.1186/1743-422X-7-156
_version_ 1782184714284564480
author Wang, Lei
Liu, Longding
Che, Yanchun
Wang, Lichun
Jiang, Li
Dong, Chenghong
Zhang, Ying
Li, Qihan
author_facet Wang, Lei
Liu, Longding
Che, Yanchun
Wang, Lichun
Jiang, Li
Dong, Chenghong
Zhang, Ying
Li, Qihan
author_sort Wang, Lei
collection PubMed
description HSV-1 viral capsid maturation and egress from the nucleus constitutes a self-controlled process of interactions between host cytoplasmic membrane proteins and viral capsid proteins. In this study, a member of the tetraspanin superfamily, CTMP-7, was shown to physically interact with HSV-1 protein VP26, and the VP26-CTMP-7 complex was detected both in vivo and in vitro. The interaction of VP26 with CTMP-7 plays an essential role in normal HSV-1 replication. Additionally, analysis of a recombinant virus HSV-1-UG showed that mutating VP26 resulted in a decreased viral replication rate and in aggregation of viral mutant capsids in the nucleus. Together, our data support the notion that biological events mediated by a VP26 - CTMP-7 interaction aid in viral capsid enveloping and egress from the cell during the HSV-1 infectious process.
format Text
id pubmed-2913958
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-29139582010-08-03 Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein Wang, Lei Liu, Longding Che, Yanchun Wang, Lichun Jiang, Li Dong, Chenghong Zhang, Ying Li, Qihan Virol J Research HSV-1 viral capsid maturation and egress from the nucleus constitutes a self-controlled process of interactions between host cytoplasmic membrane proteins and viral capsid proteins. In this study, a member of the tetraspanin superfamily, CTMP-7, was shown to physically interact with HSV-1 protein VP26, and the VP26-CTMP-7 complex was detected both in vivo and in vitro. The interaction of VP26 with CTMP-7 plays an essential role in normal HSV-1 replication. Additionally, analysis of a recombinant virus HSV-1-UG showed that mutating VP26 resulted in a decreased viral replication rate and in aggregation of viral mutant capsids in the nucleus. Together, our data support the notion that biological events mediated by a VP26 - CTMP-7 interaction aid in viral capsid enveloping and egress from the cell during the HSV-1 infectious process. BioMed Central 2010-07-14 /pmc/articles/PMC2913958/ /pubmed/20630051 http://dx.doi.org/10.1186/1743-422X-7-156 Text en Copyright ©2010 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Wang, Lei
Liu, Longding
Che, Yanchun
Wang, Lichun
Jiang, Li
Dong, Chenghong
Zhang, Ying
Li, Qihan
Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein
title Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein
title_full Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein
title_fullStr Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein
title_full_unstemmed Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein
title_short Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein
title_sort egress of hsv-1 capsid requires the interaction of vp26 and a cellular tetraspanin membrane protein
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913958/
https://www.ncbi.nlm.nih.gov/pubmed/20630051
http://dx.doi.org/10.1186/1743-422X-7-156
work_keys_str_mv AT wanglei egressofhsv1capsidrequirestheinteractionofvp26andacellulartetraspaninmembraneprotein
AT liulongding egressofhsv1capsidrequirestheinteractionofvp26andacellulartetraspaninmembraneprotein
AT cheyanchun egressofhsv1capsidrequirestheinteractionofvp26andacellulartetraspaninmembraneprotein
AT wanglichun egressofhsv1capsidrequirestheinteractionofvp26andacellulartetraspaninmembraneprotein
AT jiangli egressofhsv1capsidrequirestheinteractionofvp26andacellulartetraspaninmembraneprotein
AT dongchenghong egressofhsv1capsidrequirestheinteractionofvp26andacellulartetraspaninmembraneprotein
AT zhangying egressofhsv1capsidrequirestheinteractionofvp26andacellulartetraspaninmembraneprotein
AT liqihan egressofhsv1capsidrequirestheinteractionofvp26andacellulartetraspaninmembraneprotein

Ejemplares similares