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Hepatitis B virus genotypes and precore and core mutants in UAE patients

BACKGROUND: Knowledge of the HBV genotype with which a patient is infected is crucial information for a physician to have when planning clinical treatment for that patient. Previous studies have suggested that there are possible differences in the pathogenicity and therapeutic response of different...

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Autores principales: Alfaresi, Mubarak, Elkoush, Abida, Alshehhi, Hajer, Alzaabi, Azza, Islam, Adeel
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913959/
https://www.ncbi.nlm.nih.gov/pubmed/20633273
http://dx.doi.org/10.1186/1743-422X-7-160
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author Alfaresi, Mubarak
Elkoush, Abida
Alshehhi, Hajer
Alzaabi, Azza
Islam, Adeel
author_facet Alfaresi, Mubarak
Elkoush, Abida
Alshehhi, Hajer
Alzaabi, Azza
Islam, Adeel
author_sort Alfaresi, Mubarak
collection PubMed
description BACKGROUND: Knowledge of the HBV genotype with which a patient is infected is crucial information for a physician to have when planning clinical treatment for that patient. Previous studies have suggested that there are possible differences in the pathogenicity and therapeutic response of different HBV genotypes. However, the prevalence of the various HBV genotypes and Precore and Core mutations is unknown in the UAE. Therefore, we sought to determine the prevalence of the different HBV genotypes in the UAE population. METHODOLOGY/PRINCIPAL FINDINGS: A total of 88 HBsAg-positive patients were included in the study. A method for genotyping and subtyping HBV by partial HBsAg gene sequencing using primers that are complementary to all known genotypes was used. Precore and core region of these viruses were also sequenced in 88 patients. HBV genotype D was the most prevalent (79.5%) genotype identified in our study population, followed by genotypes A (18.2%) and C (2.3%). The following subtypes were isolated: ayw2 (80.7%), adw2 (14.8%), and adw (2.3%). The HBV-DNA viral load was higher in HBeAg-positive patients than it was in patients who were HBeAg-negative. Precore mutants were found in 51 (58.0%) of 88 patients. Mutations in the basal core promotor were found in 22 (25.3%) of 88 patients. CONCLUSION/SIGNIFICANCE: HBV infection is a major health problem in the UAE, and while genotypes B and C are the most prevalent HBV genotypes in the Asian population, our study reveals that genotype D is the predominant genotype that is present in the UAE. More patients were HBeAg-negative than were HBeAg-positive in our study sample, which could be due to the duration of infection of the included patients. Additionally, the viral loads of the HBeAg-positive patients were higher those of the HBeAg-negative patients. Analysis of nucleotide 1858 showed presence of thymine in all patients with genotypes C, and D and in a few patients with genotypes A. This nucleotide was closely related to the presence of precore mutants. Mutations in the basal core promoter were found in 22 of 88 (25.3%) samples. These mutations were more frequent in patients infected with genotype A (37.5%) and not found in patients infected with genotype C.
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spelling pubmed-29139592010-08-03 Hepatitis B virus genotypes and precore and core mutants in UAE patients Alfaresi, Mubarak Elkoush, Abida Alshehhi, Hajer Alzaabi, Azza Islam, Adeel Virol J Research BACKGROUND: Knowledge of the HBV genotype with which a patient is infected is crucial information for a physician to have when planning clinical treatment for that patient. Previous studies have suggested that there are possible differences in the pathogenicity and therapeutic response of different HBV genotypes. However, the prevalence of the various HBV genotypes and Precore and Core mutations is unknown in the UAE. Therefore, we sought to determine the prevalence of the different HBV genotypes in the UAE population. METHODOLOGY/PRINCIPAL FINDINGS: A total of 88 HBsAg-positive patients were included in the study. A method for genotyping and subtyping HBV by partial HBsAg gene sequencing using primers that are complementary to all known genotypes was used. Precore and core region of these viruses were also sequenced in 88 patients. HBV genotype D was the most prevalent (79.5%) genotype identified in our study population, followed by genotypes A (18.2%) and C (2.3%). The following subtypes were isolated: ayw2 (80.7%), adw2 (14.8%), and adw (2.3%). The HBV-DNA viral load was higher in HBeAg-positive patients than it was in patients who were HBeAg-negative. Precore mutants were found in 51 (58.0%) of 88 patients. Mutations in the basal core promotor were found in 22 (25.3%) of 88 patients. CONCLUSION/SIGNIFICANCE: HBV infection is a major health problem in the UAE, and while genotypes B and C are the most prevalent HBV genotypes in the Asian population, our study reveals that genotype D is the predominant genotype that is present in the UAE. More patients were HBeAg-negative than were HBeAg-positive in our study sample, which could be due to the duration of infection of the included patients. Additionally, the viral loads of the HBeAg-positive patients were higher those of the HBeAg-negative patients. Analysis of nucleotide 1858 showed presence of thymine in all patients with genotypes C, and D and in a few patients with genotypes A. This nucleotide was closely related to the presence of precore mutants. Mutations in the basal core promoter were found in 22 of 88 (25.3%) samples. These mutations were more frequent in patients infected with genotype A (37.5%) and not found in patients infected with genotype C. BioMed Central 2010-07-15 /pmc/articles/PMC2913959/ /pubmed/20633273 http://dx.doi.org/10.1186/1743-422X-7-160 Text en Copyright ©2010 Alfaresi et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Alfaresi, Mubarak
Elkoush, Abida
Alshehhi, Hajer
Alzaabi, Azza
Islam, Adeel
Hepatitis B virus genotypes and precore and core mutants in UAE patients
title Hepatitis B virus genotypes and precore and core mutants in UAE patients
title_full Hepatitis B virus genotypes and precore and core mutants in UAE patients
title_fullStr Hepatitis B virus genotypes and precore and core mutants in UAE patients
title_full_unstemmed Hepatitis B virus genotypes and precore and core mutants in UAE patients
title_short Hepatitis B virus genotypes and precore and core mutants in UAE patients
title_sort hepatitis b virus genotypes and precore and core mutants in uae patients
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2913959/
https://www.ncbi.nlm.nih.gov/pubmed/20633273
http://dx.doi.org/10.1186/1743-422X-7-160
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