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Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay

It has been shown by a number of authors that the radiosensitivity of peripheral blood mononuclear cells (PBMC) is higher in cancer patients compared to healthy donors, which is interpreted as a sign of genomic instability. PBMC are composed of different cell subpopulations which are differently rad...

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Autores principales: Lisowska, Halina, Deperas-Kaminska, Marta, Haghdoost, Siamak, Parmryd, Ingela, Wojcik, Andrzej
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914012/
https://www.ncbi.nlm.nih.gov/pubmed/20678258
http://dx.doi.org/10.1186/2041-9414-1-8
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author Lisowska, Halina
Deperas-Kaminska, Marta
Haghdoost, Siamak
Parmryd, Ingela
Wojcik, Andrzej
author_facet Lisowska, Halina
Deperas-Kaminska, Marta
Haghdoost, Siamak
Parmryd, Ingela
Wojcik, Andrzej
author_sort Lisowska, Halina
collection PubMed
description It has been shown by a number of authors that the radiosensitivity of peripheral blood mononuclear cells (PBMC) is higher in cancer patients compared to healthy donors, which is interpreted as a sign of genomic instability. PBMC are composed of different cell subpopulations which are differently radiosensitive and the difference between cancer patients and healthy donors could also be due to different composition of their PBMC pools. Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy. Their abundance is frequently reduced in cancer patients so should their sensitivity to radiation be lower than that of other T-lymphocytes, this could, at least partly explain the low radiosensitivity of PBMC from healthy individuals compared to cancer patients. The present investigation was carried out to test this. Using the alkaline comet assay we analysed the level of DNA damage and repair in isolated γδ T-lymphocytes, pan T-lymphocytes and in total PBMC exposed in vitro to gamma radiation. We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations. This is the first study that addresses the question of sensitivity to radiation of gamma-delta T-cells.
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spelling pubmed-29140122010-08-03 Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay Lisowska, Halina Deperas-Kaminska, Marta Haghdoost, Siamak Parmryd, Ingela Wojcik, Andrzej Genome Integr Research It has been shown by a number of authors that the radiosensitivity of peripheral blood mononuclear cells (PBMC) is higher in cancer patients compared to healthy donors, which is interpreted as a sign of genomic instability. PBMC are composed of different cell subpopulations which are differently radiosensitive and the difference between cancer patients and healthy donors could also be due to different composition of their PBMC pools. Gamma-delta T-lymphocytes play an important role in immunosurveillance and are promising cells for immunotherapy. Their abundance is frequently reduced in cancer patients so should their sensitivity to radiation be lower than that of other T-lymphocytes, this could, at least partly explain the low radiosensitivity of PBMC from healthy individuals compared to cancer patients. The present investigation was carried out to test this. Using the alkaline comet assay we analysed the level of DNA damage and repair in isolated γδ T-lymphocytes, pan T-lymphocytes and in total PBMC exposed in vitro to gamma radiation. We found no difference in the level of DNA damage and the capacity of DNA repair between the T cell populations. This is the first study that addresses the question of sensitivity to radiation of gamma-delta T-cells. BioMed Central 2010-06-08 /pmc/articles/PMC2914012/ /pubmed/20678258 http://dx.doi.org/10.1186/2041-9414-1-8 Text en Copyright ©2010 Lisowska et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research
Lisowska, Halina
Deperas-Kaminska, Marta
Haghdoost, Siamak
Parmryd, Ingela
Wojcik, Andrzej
Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay
title Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay
title_full Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay
title_fullStr Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay
title_full_unstemmed Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay
title_short Radiation-induced DNA damage and repair in human γδ and αβ T-lymphocytes analysed by the alkaline comet assay
title_sort radiation-induced dna damage and repair in human γδ and αβ t-lymphocytes analysed by the alkaline comet assay
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914012/
https://www.ncbi.nlm.nih.gov/pubmed/20678258
http://dx.doi.org/10.1186/2041-9414-1-8
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