Rationally improving LOV domain–based photoswitches

Genetically-encoded protein photosensors, including the LOV (light, oxygen, voltage) domain, are promising tools for engineering optical control of cellular behavior. We are only beginning to understand how to couple these light detectors to effectors of choice. We report a method that increases the...

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Detalles Bibliográficos
Autores principales: Strickland, Devin, Yao, Xiaolan, Gawlak, Grzegorz, Rosen, Michael K., Gardner, Kevin H., Sosnick, Tobin R.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914111/
https://www.ncbi.nlm.nih.gov/pubmed/20562867
http://dx.doi.org/10.1038/nmeth.1473
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author Strickland, Devin
Yao, Xiaolan
Gawlak, Grzegorz
Rosen, Michael K.
Gardner, Kevin H.
Sosnick, Tobin R.
author_facet Strickland, Devin
Yao, Xiaolan
Gawlak, Grzegorz
Rosen, Michael K.
Gardner, Kevin H.
Sosnick, Tobin R.
author_sort Strickland, Devin
collection PubMed
description Genetically-encoded protein photosensors, including the LOV (light, oxygen, voltage) domain, are promising tools for engineering optical control of cellular behavior. We are only beginning to understand how to couple these light detectors to effectors of choice. We report a method that increases the dynamic range of an artificial photoswitch based on the LOV2 domain of A. sativa phototropin1 (AsLOV2). This approach can potentially be used to improve many AsLOV2-based photoswitches.
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spelling pubmed-29141112011-02-01 Rationally improving LOV domain–based photoswitches Strickland, Devin Yao, Xiaolan Gawlak, Grzegorz Rosen, Michael K. Gardner, Kevin H. Sosnick, Tobin R. Nat Methods Article Genetically-encoded protein photosensors, including the LOV (light, oxygen, voltage) domain, are promising tools for engineering optical control of cellular behavior. We are only beginning to understand how to couple these light detectors to effectors of choice. We report a method that increases the dynamic range of an artificial photoswitch based on the LOV2 domain of A. sativa phototropin1 (AsLOV2). This approach can potentially be used to improve many AsLOV2-based photoswitches. 2010-06-20 2010-08 /pmc/articles/PMC2914111/ /pubmed/20562867 http://dx.doi.org/10.1038/nmeth.1473 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Strickland, Devin
Yao, Xiaolan
Gawlak, Grzegorz
Rosen, Michael K.
Gardner, Kevin H.
Sosnick, Tobin R.
Rationally improving LOV domain–based photoswitches
title Rationally improving LOV domain–based photoswitches
title_full Rationally improving LOV domain–based photoswitches
title_fullStr Rationally improving LOV domain–based photoswitches
title_full_unstemmed Rationally improving LOV domain–based photoswitches
title_short Rationally improving LOV domain–based photoswitches
title_sort rationally improving lov domain–based photoswitches
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914111/
https://www.ncbi.nlm.nih.gov/pubmed/20562867
http://dx.doi.org/10.1038/nmeth.1473
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AT rosenmichaelk rationallyimprovinglovdomainbasedphotoswitches
AT gardnerkevinh rationallyimprovinglovdomainbasedphotoswitches
AT sosnicktobinr rationallyimprovinglovdomainbasedphotoswitches

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