Cargando…
Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs
Obtaining suitable seed viruses for influenza vaccines poses a challenge for public health authorities and manufacturers. We used reverse genetics to generate vaccine seed-compatible viruses from the 2009 pandemic swine-origin influenza virus. Comparison of viruses recovered with variations in resid...
| Autores principales: | , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914672/ https://www.ncbi.nlm.nih.gov/pubmed/20630098 http://dx.doi.org/10.1186/1743-422X-7-157 |
| _version_ | 1782184777421422592 |
|---|---|
| author | Suphaphiphat, Pirada Franti, Michael Hekele, Armin Lilja, Anders Spencer, Terika Settembre, Ethan Palmer, Gene Crotta, Stefania Tuccino, Annunziata B Keiner, Bjoern Trusheim, Heidi Balabanis, Kara Sackal, Melissa Rothfeder, Mithra Mandl, Christian W Dormitzer, Philip R Mason, Peter W |
| author_facet | Suphaphiphat, Pirada Franti, Michael Hekele, Armin Lilja, Anders Spencer, Terika Settembre, Ethan Palmer, Gene Crotta, Stefania Tuccino, Annunziata B Keiner, Bjoern Trusheim, Heidi Balabanis, Kara Sackal, Melissa Rothfeder, Mithra Mandl, Christian W Dormitzer, Philip R Mason, Peter W |
| author_sort | Suphaphiphat, Pirada |
| collection | PubMed |
| description | Obtaining suitable seed viruses for influenza vaccines poses a challenge for public health authorities and manufacturers. We used reverse genetics to generate vaccine seed-compatible viruses from the 2009 pandemic swine-origin influenza virus. Comparison of viruses recovered with variations in residues 186 and 194 (based on the H3 numbering system) of the viral hemagglutinin showed that these viruses differed with respect to their ability to grow in eggs and cultured cells. Thus, we have demonstrated that molecular cloning of members of a quasispecies can help in selection of seed viruses for vaccine manufacture. |
| format | Text |
| id | pubmed-2914672 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2010 |
| publisher | BioMed Central |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-29146722010-08-04 Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs Suphaphiphat, Pirada Franti, Michael Hekele, Armin Lilja, Anders Spencer, Terika Settembre, Ethan Palmer, Gene Crotta, Stefania Tuccino, Annunziata B Keiner, Bjoern Trusheim, Heidi Balabanis, Kara Sackal, Melissa Rothfeder, Mithra Mandl, Christian W Dormitzer, Philip R Mason, Peter W Virol J Short Report Obtaining suitable seed viruses for influenza vaccines poses a challenge for public health authorities and manufacturers. We used reverse genetics to generate vaccine seed-compatible viruses from the 2009 pandemic swine-origin influenza virus. Comparison of viruses recovered with variations in residues 186 and 194 (based on the H3 numbering system) of the viral hemagglutinin showed that these viruses differed with respect to their ability to grow in eggs and cultured cells. Thus, we have demonstrated that molecular cloning of members of a quasispecies can help in selection of seed viruses for vaccine manufacture. BioMed Central 2010-07-14 /pmc/articles/PMC2914672/ /pubmed/20630098 http://dx.doi.org/10.1186/1743-422X-7-157 Text en Copyright ©2010 Suphaphiphat et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
| spellingShingle | Short Report Suphaphiphat, Pirada Franti, Michael Hekele, Armin Lilja, Anders Spencer, Terika Settembre, Ethan Palmer, Gene Crotta, Stefania Tuccino, Annunziata B Keiner, Bjoern Trusheim, Heidi Balabanis, Kara Sackal, Melissa Rothfeder, Mithra Mandl, Christian W Dormitzer, Philip R Mason, Peter W Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs |
| title | Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs |
| title_full | Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs |
| title_fullStr | Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs |
| title_full_unstemmed | Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs |
| title_short | Mutations at positions 186 and 194 in the HA gene of the 2009 H1N1 pandemic influenza virus improve replication in cell culture and eggs |
| title_sort | mutations at positions 186 and 194 in the ha gene of the 2009 h1n1 pandemic influenza virus improve replication in cell culture and eggs |
| topic | Short Report |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914672/ https://www.ncbi.nlm.nih.gov/pubmed/20630098 http://dx.doi.org/10.1186/1743-422X-7-157 |
| work_keys_str_mv | AT suphaphiphatpirada mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT frantimichael mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT hekelearmin mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT liljaanders mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT spencerterika mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT settembreethan mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT palmergene mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT crottastefania mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT tuccinoannunziatab mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT keinerbjoern mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT trusheimheidi mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT balabaniskara mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT sackalmelissa mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT rothfedermithra mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT mandlchristianw mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT dormitzerphilipr mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs AT masonpeterw mutationsatpositions186and194inthehageneofthe2009h1n1pandemicinfluenzavirusimprovereplicationincellcultureandeggs |