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Nanoparticulate Radiolabelled Quinolines Detect Amyloid Plaques in Mouse Models of Alzheimer's Disease
Detecting aggregated amyloid peptides (Aβ plaques) presents targets for developing biomarkers of Alzheimer's disease (AD). Polymeric n-butyl-2-cyanoacrylate (PBCA) nanoparticles (NPs) were encapsulated with radiolabelled amyloid affinity (125)I-clioquinol (CQ, 5-chloro-7-iodo-8-hydroxyquinoline...
Autores principales: | , , , , |
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Formato: | Texto |
Lenguaje: | English |
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SAGE-Hindawi Access to Research
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915660/ https://www.ncbi.nlm.nih.gov/pubmed/20721294 http://dx.doi.org/10.4061/2009/481031 |
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author | Roney, Celeste A. Arora, Veera Kulkarni, Padmakar V. Antich, Peter P. Bonte, Frederick J. |
author_facet | Roney, Celeste A. Arora, Veera Kulkarni, Padmakar V. Antich, Peter P. Bonte, Frederick J. |
author_sort | Roney, Celeste A. |
collection | PubMed |
description | Detecting aggregated amyloid peptides (Aβ plaques) presents targets for developing biomarkers of Alzheimer's disease (AD). Polymeric n-butyl-2-cyanoacrylate (PBCA) nanoparticles (NPs) were encapsulated with radiolabelled amyloid affinity (125)I-clioquinol (CQ, 5-chloro-7-iodo-8-hydroxyquinoline) as in vivo probes. (125)I-CQ-PBCA NPs crossed the BBB (2.3 ± 0.9 ID/g) (P < .05) in the WT mouse (N = 210), compared to (125)I-CQ (1.0 ± 0.4 ID/g). (125)I-CQ-PBCA NP brain uptake increased in AD transgenic mice (APP/PS1) versus WT (N = 38; 2.54 × 10(5) ± 5.31 × 10(4) DLU/mm(2); versus 1.98 × 10(5) ± 2.22 × 10(4) DLU/mm(2)) and in APP/PS1/Tau. Brain increases were in mice intracranially injected with aggregated Aβ (42) peptide (N = 17; 7.19 × 10(5) ± 1.25 × 10(5) DLU/mm(2)), versus WT (6.07 × 10(5) ± 7.47 × 10(4) DLU/mm(2)). Storage phosphor imaging and histopathological staining of the plaques, Fe(2+) and Cu(2+), validated results. (125)I-CQ-PBCA NPs have specificity for Aβ in vitro and in vivo and are promising as in vivo SPECT ((123)I), or PET ((124)I) amyloid imaging agents. |
format | Text |
id | pubmed-2915660 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | SAGE-Hindawi Access to Research |
record_format | MEDLINE/PubMed |
spelling | pubmed-29156602010-08-18 Nanoparticulate Radiolabelled Quinolines Detect Amyloid Plaques in Mouse Models of Alzheimer's Disease Roney, Celeste A. Arora, Veera Kulkarni, Padmakar V. Antich, Peter P. Bonte, Frederick J. Int J Alzheimers Dis Research Article Detecting aggregated amyloid peptides (Aβ plaques) presents targets for developing biomarkers of Alzheimer's disease (AD). Polymeric n-butyl-2-cyanoacrylate (PBCA) nanoparticles (NPs) were encapsulated with radiolabelled amyloid affinity (125)I-clioquinol (CQ, 5-chloro-7-iodo-8-hydroxyquinoline) as in vivo probes. (125)I-CQ-PBCA NPs crossed the BBB (2.3 ± 0.9 ID/g) (P < .05) in the WT mouse (N = 210), compared to (125)I-CQ (1.0 ± 0.4 ID/g). (125)I-CQ-PBCA NP brain uptake increased in AD transgenic mice (APP/PS1) versus WT (N = 38; 2.54 × 10(5) ± 5.31 × 10(4) DLU/mm(2); versus 1.98 × 10(5) ± 2.22 × 10(4) DLU/mm(2)) and in APP/PS1/Tau. Brain increases were in mice intracranially injected with aggregated Aβ (42) peptide (N = 17; 7.19 × 10(5) ± 1.25 × 10(5) DLU/mm(2)), versus WT (6.07 × 10(5) ± 7.47 × 10(4) DLU/mm(2)). Storage phosphor imaging and histopathological staining of the plaques, Fe(2+) and Cu(2+), validated results. (125)I-CQ-PBCA NPs have specificity for Aβ in vitro and in vivo and are promising as in vivo SPECT ((123)I), or PET ((124)I) amyloid imaging agents. SAGE-Hindawi Access to Research 2010-01-27 /pmc/articles/PMC2915660/ /pubmed/20721294 http://dx.doi.org/10.4061/2009/481031 Text en Copyright © 2009 Celeste A. Roney et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Roney, Celeste A. Arora, Veera Kulkarni, Padmakar V. Antich, Peter P. Bonte, Frederick J. Nanoparticulate Radiolabelled Quinolines Detect Amyloid Plaques in Mouse Models of Alzheimer's Disease |
title | Nanoparticulate Radiolabelled Quinolines Detect Amyloid Plaques in Mouse Models of Alzheimer's Disease |
title_full | Nanoparticulate Radiolabelled Quinolines Detect Amyloid Plaques in Mouse Models of Alzheimer's Disease |
title_fullStr | Nanoparticulate Radiolabelled Quinolines Detect Amyloid Plaques in Mouse Models of Alzheimer's Disease |
title_full_unstemmed | Nanoparticulate Radiolabelled Quinolines Detect Amyloid Plaques in Mouse Models of Alzheimer's Disease |
title_short | Nanoparticulate Radiolabelled Quinolines Detect Amyloid Plaques in Mouse Models of Alzheimer's Disease |
title_sort | nanoparticulate radiolabelled quinolines detect amyloid plaques in mouse models of alzheimer's disease |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915660/ https://www.ncbi.nlm.nih.gov/pubmed/20721294 http://dx.doi.org/10.4061/2009/481031 |
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