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Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries)
Eight reproductive rams with no prior reproductive disease were distributed into three groups of infection with T. gondii: GI, 3 rams, 2.0 × 10(5) P strain oocysts; GII, 3 rams, 1.0 × 10(6) RH strain tachyzoites; GIII, 2 control rams. Clinical parameters were measured and serological evaluations (II...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Hindawi Publishing Corporation
2009
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915749/ https://www.ncbi.nlm.nih.gov/pubmed/20721328 http://dx.doi.org/10.1155/2009/602803 |
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author | Lopes, Welber Daniel Zanetti da Costa, Alvimar José Santana, Luis Fernando dos Santos, Ricardo Silva Rossanese, Walter Matheus Lopes, Wilton Carlos Zanetti Costa, Gustavo Henrique Nogueira Sakamoto, Cláudio Alessandro dos Santos, Thais Rabelo |
author_facet | Lopes, Welber Daniel Zanetti da Costa, Alvimar José Santana, Luis Fernando dos Santos, Ricardo Silva Rossanese, Walter Matheus Lopes, Wilton Carlos Zanetti Costa, Gustavo Henrique Nogueira Sakamoto, Cláudio Alessandro dos Santos, Thais Rabelo |
author_sort | Lopes, Welber Daniel Zanetti |
collection | PubMed |
description | Eight reproductive rams with no prior reproductive disease were distributed into three groups of infection with T. gondii: GI, 3 rams, 2.0 × 10(5) P strain oocysts; GII, 3 rams, 1.0 × 10(6) RH strain tachyzoites; GIII, 2 control rams. Clinical parameters were measured and serological evaluations (IIF) were performed. Presence of the parasite in the semen was investigated by PCR and bioassay techniques. The rams presented clinical alterations (hyperthermia and apathy) related to toxoplasmosis in both groups infected with Toxoplasma gondii. All the inoculated rams responded to antigenic stimulus, producing antibodies against T. gondii from postinoculation day 5 onwards. In ovine groups I and II, the greatest titers observed were 1 : 4096 and 1 : 8192, respectively. In semen samples collected from these two groups, the presence of T. gondii was detected by bioassay and PCR. This coccidian was isolated (bioassay and PCR) in tissue pools (testicles, epididymis, seminal vesicle, and prostrate) from two rams infected presenting oocysts and in one presenting tachyzoites. |
format | Text |
id | pubmed-2915749 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2009 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-29157492010-08-18 Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries) Lopes, Welber Daniel Zanetti da Costa, Alvimar José Santana, Luis Fernando dos Santos, Ricardo Silva Rossanese, Walter Matheus Lopes, Wilton Carlos Zanetti Costa, Gustavo Henrique Nogueira Sakamoto, Cláudio Alessandro dos Santos, Thais Rabelo J Parasitol Res Research Article Eight reproductive rams with no prior reproductive disease were distributed into three groups of infection with T. gondii: GI, 3 rams, 2.0 × 10(5) P strain oocysts; GII, 3 rams, 1.0 × 10(6) RH strain tachyzoites; GIII, 2 control rams. Clinical parameters were measured and serological evaluations (IIF) were performed. Presence of the parasite in the semen was investigated by PCR and bioassay techniques. The rams presented clinical alterations (hyperthermia and apathy) related to toxoplasmosis in both groups infected with Toxoplasma gondii. All the inoculated rams responded to antigenic stimulus, producing antibodies against T. gondii from postinoculation day 5 onwards. In ovine groups I and II, the greatest titers observed were 1 : 4096 and 1 : 8192, respectively. In semen samples collected from these two groups, the presence of T. gondii was detected by bioassay and PCR. This coccidian was isolated (bioassay and PCR) in tissue pools (testicles, epididymis, seminal vesicle, and prostrate) from two rams infected presenting oocysts and in one presenting tachyzoites. Hindawi Publishing Corporation 2009 2009-05-27 /pmc/articles/PMC2915749/ /pubmed/20721328 http://dx.doi.org/10.1155/2009/602803 Text en Copyright © 2009 Welber Daniel Zanetti Lopes et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Lopes, Welber Daniel Zanetti da Costa, Alvimar José Santana, Luis Fernando dos Santos, Ricardo Silva Rossanese, Walter Matheus Lopes, Wilton Carlos Zanetti Costa, Gustavo Henrique Nogueira Sakamoto, Cláudio Alessandro dos Santos, Thais Rabelo Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries) |
title | Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries) |
title_full | Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries) |
title_fullStr | Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries) |
title_full_unstemmed | Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries) |
title_short | Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries) |
title_sort | aspects of toxoplasma infection on the reproductive system of experimentally infected rams (ovis aries) |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915749/ https://www.ncbi.nlm.nih.gov/pubmed/20721328 http://dx.doi.org/10.1155/2009/602803 |
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