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Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries)

Eight reproductive rams with no prior reproductive disease were distributed into three groups of infection with T. gondii: GI, 3 rams, 2.0 × 10(5) P strain oocysts; GII, 3 rams, 1.0 × 10(6) RH strain tachyzoites; GIII, 2 control rams. Clinical parameters were measured and serological evaluations (II...

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Autores principales: Lopes, Welber Daniel Zanetti, da Costa, Alvimar José, Santana, Luis Fernando, dos Santos, Ricardo Silva, Rossanese, Walter Matheus, Lopes, Wilton Carlos Zanetti, Costa, Gustavo Henrique Nogueira, Sakamoto, Cláudio Alessandro, dos Santos, Thais Rabelo
Formato: Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2009
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915749/
https://www.ncbi.nlm.nih.gov/pubmed/20721328
http://dx.doi.org/10.1155/2009/602803
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author Lopes, Welber Daniel Zanetti
da Costa, Alvimar José
Santana, Luis Fernando
dos Santos, Ricardo Silva
Rossanese, Walter Matheus
Lopes, Wilton Carlos Zanetti
Costa, Gustavo Henrique Nogueira
Sakamoto, Cláudio Alessandro
dos Santos, Thais Rabelo
author_facet Lopes, Welber Daniel Zanetti
da Costa, Alvimar José
Santana, Luis Fernando
dos Santos, Ricardo Silva
Rossanese, Walter Matheus
Lopes, Wilton Carlos Zanetti
Costa, Gustavo Henrique Nogueira
Sakamoto, Cláudio Alessandro
dos Santos, Thais Rabelo
author_sort Lopes, Welber Daniel Zanetti
collection PubMed
description Eight reproductive rams with no prior reproductive disease were distributed into three groups of infection with T. gondii: GI, 3 rams, 2.0 × 10(5) P strain oocysts; GII, 3 rams, 1.0 × 10(6) RH strain tachyzoites; GIII, 2 control rams. Clinical parameters were measured and serological evaluations (IIF) were performed. Presence of the parasite in the semen was investigated by PCR and bioassay techniques. The rams presented clinical alterations (hyperthermia and apathy) related to toxoplasmosis in both groups infected with Toxoplasma gondii. All the inoculated rams responded to antigenic stimulus, producing antibodies against T. gondii from postinoculation day 5 onwards. In ovine groups I and II, the greatest titers observed were 1 : 4096 and 1 : 8192, respectively. In semen samples collected from these two groups, the presence of T. gondii was detected by bioassay and PCR. This coccidian was isolated (bioassay and PCR) in tissue pools (testicles, epididymis, seminal vesicle, and prostrate) from two rams infected presenting oocysts and in one presenting tachyzoites.
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spelling pubmed-29157492010-08-18 Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries) Lopes, Welber Daniel Zanetti da Costa, Alvimar José Santana, Luis Fernando dos Santos, Ricardo Silva Rossanese, Walter Matheus Lopes, Wilton Carlos Zanetti Costa, Gustavo Henrique Nogueira Sakamoto, Cláudio Alessandro dos Santos, Thais Rabelo J Parasitol Res Research Article Eight reproductive rams with no prior reproductive disease were distributed into three groups of infection with T. gondii: GI, 3 rams, 2.0 × 10(5) P strain oocysts; GII, 3 rams, 1.0 × 10(6) RH strain tachyzoites; GIII, 2 control rams. Clinical parameters were measured and serological evaluations (IIF) were performed. Presence of the parasite in the semen was investigated by PCR and bioassay techniques. The rams presented clinical alterations (hyperthermia and apathy) related to toxoplasmosis in both groups infected with Toxoplasma gondii. All the inoculated rams responded to antigenic stimulus, producing antibodies against T. gondii from postinoculation day 5 onwards. In ovine groups I and II, the greatest titers observed were 1 : 4096 and 1 : 8192, respectively. In semen samples collected from these two groups, the presence of T. gondii was detected by bioassay and PCR. This coccidian was isolated (bioassay and PCR) in tissue pools (testicles, epididymis, seminal vesicle, and prostrate) from two rams infected presenting oocysts and in one presenting tachyzoites. Hindawi Publishing Corporation 2009 2009-05-27 /pmc/articles/PMC2915749/ /pubmed/20721328 http://dx.doi.org/10.1155/2009/602803 Text en Copyright © 2009 Welber Daniel Zanetti Lopes et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Lopes, Welber Daniel Zanetti
da Costa, Alvimar José
Santana, Luis Fernando
dos Santos, Ricardo Silva
Rossanese, Walter Matheus
Lopes, Wilton Carlos Zanetti
Costa, Gustavo Henrique Nogueira
Sakamoto, Cláudio Alessandro
dos Santos, Thais Rabelo
Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries)
title Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries)
title_full Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries)
title_fullStr Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries)
title_full_unstemmed Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries)
title_short Aspects of Toxoplasma Infection on the Reproductive System of Experimentally Infected Rams (Ovis Aries)
title_sort aspects of toxoplasma infection on the reproductive system of experimentally infected rams (ovis aries)
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2915749/
https://www.ncbi.nlm.nih.gov/pubmed/20721328
http://dx.doi.org/10.1155/2009/602803
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