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Optimus Primer: A PCR enrichment primer design program for next-generation sequencing of human exonic regions
BACKGROUND: Polymerase chain reaction (PCR) remains a simple, flexible, and inexpensive method for enriching genomic regions of interest for next-generation sequencing. In order to utilize PCR in this context, a major challenge facing researchers is how to generate a very large number of functional...
| Autores principales: | , , , , , , , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
BioMed Central
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916007/ https://www.ncbi.nlm.nih.gov/pubmed/20609249 http://dx.doi.org/10.1186/1756-0500-3-185 |