Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines

Recent studies have suggested that ribosomal protein S12 modulates 16S rRNA function and susceptibility to 2-deoxystreptamine aminoglycosides. To study whether the non-restrictive K42R mutation in RpsL affects 2-deoxystreptamine susceptibility in Mycobacterium smegmatis, we studied the drug suscepti...

Descripción completa

Detalles Bibliográficos
Autores principales: Kalapala, Sarath K., Hobbie, Sven N., Böttger, Erik C., Shcherbakov, Dmitry
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916820/
https://www.ncbi.nlm.nih.gov/pubmed/20700526
http://dx.doi.org/10.1371/journal.pone.0011960
_version_ 1782185015531012096
author Kalapala, Sarath K.
Hobbie, Sven N.
Böttger, Erik C.
Shcherbakov, Dmitry
author_facet Kalapala, Sarath K.
Hobbie, Sven N.
Böttger, Erik C.
Shcherbakov, Dmitry
author_sort Kalapala, Sarath K.
collection PubMed
description Recent studies have suggested that ribosomal protein S12 modulates 16S rRNA function and susceptibility to 2-deoxystreptamine aminoglycosides. To study whether the non-restrictive K42R mutation in RpsL affects 2-deoxystreptamine susceptibility in Mycobacterium smegmatis, we studied the drug susceptibility pattern of various mutants with genetic alterations in the 16S rRNA decoding A-site in the context of wild-type and mutant protein S12. RpsL K42R substitution was found not to affect the drug resistance pattern associated with mutational alterations in 16S rRNA H44.
format Text
id pubmed-2916820
institution National Center for Biotechnology Information
language English
publishDate 2010
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-29168202010-08-10 Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines Kalapala, Sarath K. Hobbie, Sven N. Böttger, Erik C. Shcherbakov, Dmitry PLoS One Research Article Recent studies have suggested that ribosomal protein S12 modulates 16S rRNA function and susceptibility to 2-deoxystreptamine aminoglycosides. To study whether the non-restrictive K42R mutation in RpsL affects 2-deoxystreptamine susceptibility in Mycobacterium smegmatis, we studied the drug susceptibility pattern of various mutants with genetic alterations in the 16S rRNA decoding A-site in the context of wild-type and mutant protein S12. RpsL K42R substitution was found not to affect the drug resistance pattern associated with mutational alterations in 16S rRNA H44. Public Library of Science 2010-08-05 /pmc/articles/PMC2916820/ /pubmed/20700526 http://dx.doi.org/10.1371/journal.pone.0011960 Text en Kalapala et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Kalapala, Sarath K.
Hobbie, Sven N.
Böttger, Erik C.
Shcherbakov, Dmitry
Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines
title Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines
title_full Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines
title_fullStr Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines
title_full_unstemmed Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines
title_short Mutation K42R in Ribosomal Protein S12 Does Not Affect Susceptibility of Mycobacterium smegmatis 16S rRNA A-Site Mutants to 2-Deoxystreptamines
title_sort mutation k42r in ribosomal protein s12 does not affect susceptibility of mycobacterium smegmatis 16s rrna a-site mutants to 2-deoxystreptamines
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2916820/
https://www.ncbi.nlm.nih.gov/pubmed/20700526
http://dx.doi.org/10.1371/journal.pone.0011960
work_keys_str_mv AT kalapalasarathk mutationk42rinribosomalproteins12doesnotaffectsusceptibilityofmycobacteriumsmegmatis16srrnaasitemutantsto2deoxystreptamines
AT hobbiesvenn mutationk42rinribosomalproteins12doesnotaffectsusceptibilityofmycobacteriumsmegmatis16srrnaasitemutantsto2deoxystreptamines
AT bottgererikc mutationk42rinribosomalproteins12doesnotaffectsusceptibilityofmycobacteriumsmegmatis16srrnaasitemutantsto2deoxystreptamines
AT shcherbakovdmitry mutationk42rinribosomalproteins12doesnotaffectsusceptibilityofmycobacteriumsmegmatis16srrnaasitemutantsto2deoxystreptamines

Ejemplares similares