High-Throughput SuperSAGE for Digital Gene Expression Analysis of Multiple Samples Using Next Generation Sequencing

We established a protocol of the SuperSAGE technology combined with next-generation sequencing, coined “High-Throughput (HT-) SuperSAGE”. SuperSAGE is a method of digital gene expression profiling that allows isolation of 26-bp tag fragments from expressed transcripts. In the present protocol, index...

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Detalles Bibliográficos
Autores principales: Matsumura, Hideo, Yoshida, Kentaro, Luo, Shujun, Kimura, Eiji, Fujibe, Takahiro, Albertyn, Zayed, Barrero, Roberto A., Krüger, Detlev H., Kahl, Günter, Schroth, Gary P., Terauchi, Ryohei
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2917361/
https://www.ncbi.nlm.nih.gov/pubmed/20700453
http://dx.doi.org/10.1371/journal.pone.0012010

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