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Silenced yeast chromatin is maintained by Sir2 in preference to permitting histone acetylations for efficient NER

Very little is currently known about how nucleotide excision repair (NER) functions at the ends of chromosomes. To examine this, we introduced the URA3 gene into either transcriptionally active or repressed subtelomeric regions of the yeast genome. This enabled us to examine the repair of ultraviole...

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Autores principales: Irizar, Agurtzane, Yu, Yachuan, Reed, Simon H., Louis, Edward J., Waters, Raymond
Formato: Texto
Lenguaje:English
Publicado: Oxford University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2919727/
https://www.ncbi.nlm.nih.gov/pubmed/20385597
http://dx.doi.org/10.1093/nar/gkq242
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author Irizar, Agurtzane
Yu, Yachuan
Reed, Simon H.
Louis, Edward J.
Waters, Raymond
author_facet Irizar, Agurtzane
Yu, Yachuan
Reed, Simon H.
Louis, Edward J.
Waters, Raymond
author_sort Irizar, Agurtzane
collection PubMed
description Very little is currently known about how nucleotide excision repair (NER) functions at the ends of chromosomes. To examine this, we introduced the URA3 gene into either transcriptionally active or repressed subtelomeric regions of the yeast genome. This enabled us to examine the repair of ultraviolet (UV)-induced cyclobutane pyrimidine dimers (CPDs) in identical sequences under both circumstances. We found that NER is significantly more efficient in the non-repressed subtelomere than the repressed one. At the non-repressed subtelomere, UV radiation stimulates both histones H3 and H4 acetylation in a similar fashion to that seen at other regions of the yeast genome. These modifications occur regardless of the presence of the Sir2 histone deacetylase. On the other hand, at the repressed subtelomere, where repair is much less efficient, UV radiation is unable to stimulate histone H4 or H3 acetylation in the presence of Sir2. In the absence of Sir2 both of these UV-induced modifications are detected, resulting in a significant increase in NER efficiency in the region. Our experiments reveal that there are instances in the yeast genome where the maintenance of the existing chromatin structures dominates over the action of chromatin modifications associated with efficient NER.
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spelling pubmed-29197272010-08-11 Silenced yeast chromatin is maintained by Sir2 in preference to permitting histone acetylations for efficient NER Irizar, Agurtzane Yu, Yachuan Reed, Simon H. Louis, Edward J. Waters, Raymond Nucleic Acids Res Gene Regulation, Chromatin and Epigenetics Very little is currently known about how nucleotide excision repair (NER) functions at the ends of chromosomes. To examine this, we introduced the URA3 gene into either transcriptionally active or repressed subtelomeric regions of the yeast genome. This enabled us to examine the repair of ultraviolet (UV)-induced cyclobutane pyrimidine dimers (CPDs) in identical sequences under both circumstances. We found that NER is significantly more efficient in the non-repressed subtelomere than the repressed one. At the non-repressed subtelomere, UV radiation stimulates both histones H3 and H4 acetylation in a similar fashion to that seen at other regions of the yeast genome. These modifications occur regardless of the presence of the Sir2 histone deacetylase. On the other hand, at the repressed subtelomere, where repair is much less efficient, UV radiation is unable to stimulate histone H4 or H3 acetylation in the presence of Sir2. In the absence of Sir2 both of these UV-induced modifications are detected, resulting in a significant increase in NER efficiency in the region. Our experiments reveal that there are instances in the yeast genome where the maintenance of the existing chromatin structures dominates over the action of chromatin modifications associated with efficient NER. Oxford University Press 2010-08 2010-04-12 /pmc/articles/PMC2919727/ /pubmed/20385597 http://dx.doi.org/10.1093/nar/gkq242 Text en © The Author(s) 2010. Published by Oxford University Press. http://creativecommons.org/licenses/by-nc/2.5 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gene Regulation, Chromatin and Epigenetics
Irizar, Agurtzane
Yu, Yachuan
Reed, Simon H.
Louis, Edward J.
Waters, Raymond
Silenced yeast chromatin is maintained by Sir2 in preference to permitting histone acetylations for efficient NER
title Silenced yeast chromatin is maintained by Sir2 in preference to permitting histone acetylations for efficient NER
title_full Silenced yeast chromatin is maintained by Sir2 in preference to permitting histone acetylations for efficient NER
title_fullStr Silenced yeast chromatin is maintained by Sir2 in preference to permitting histone acetylations for efficient NER
title_full_unstemmed Silenced yeast chromatin is maintained by Sir2 in preference to permitting histone acetylations for efficient NER
title_short Silenced yeast chromatin is maintained by Sir2 in preference to permitting histone acetylations for efficient NER
title_sort silenced yeast chromatin is maintained by sir2 in preference to permitting histone acetylations for efficient ner
topic Gene Regulation, Chromatin and Epigenetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2919727/
https://www.ncbi.nlm.nih.gov/pubmed/20385597
http://dx.doi.org/10.1093/nar/gkq242
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AT louisedwardj silencedyeastchromatinismaintainedbysir2inpreferencetopermittinghistoneacetylationsforefficientner
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