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Insect Repellents: Modulators of Mosquito Odorant Receptor Activity

BACKGROUND: DEET, 2-undecanone (2-U), IR3535 and Picaridin are widely used as insect repellents to prevent interactions between humans and many arthropods including mosquitoes. Their molecular action has only recently been studied, yielding seemingly contradictory theories including odorant-dependen...

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Autores principales: Bohbot, Jonathan D., Dickens, Joseph C.
Formato: Texto
Lenguaje:English
Publicado: Public Library of Science 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920324/
https://www.ncbi.nlm.nih.gov/pubmed/20725637
http://dx.doi.org/10.1371/journal.pone.0012138
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author Bohbot, Jonathan D.
Dickens, Joseph C.
author_facet Bohbot, Jonathan D.
Dickens, Joseph C.
author_sort Bohbot, Jonathan D.
collection PubMed
description BACKGROUND: DEET, 2-undecanone (2-U), IR3535 and Picaridin are widely used as insect repellents to prevent interactions between humans and many arthropods including mosquitoes. Their molecular action has only recently been studied, yielding seemingly contradictory theories including odorant-dependent inhibitory and odorant-independent excitatory activities on insect olfactory sensory neurons (OSNs) and odorant receptor proteins (ORs). METHODOLOGY/PRINCIPAL FINDINGS: Here we characterize the action of these repellents on two Aedes aegypti ORs, AaOR2 and AaOR8, individually co-expressed with the common co-receptor AaOR7 in Xenopus oocytes; these ORs are respectively activated by the odors indole (AaOR2) and (R)-(−)-1-octen3-ol (AaOR8), odorants used to locate oviposition sites and host animals. In the absence of odorants, DEET activates AaOR2 but not AaOR8, while 2-U activates AaOR8 but not AaOR2; IR3535 and Picaridin do not activate these ORs. In the presence of odors, DEET strongly inhibits AaOR8 but not AaOR2, while 2-U strongly inhibits AaOR2 but not AaOR8; IR3535 and Picaridin strongly inhibit both ORs. CONCLUSIONS/SIGNIFICANCE: These data demonstrate that repellents can act as olfactory agonists or antagonists, thus modulating OR activity, bringing concordance to conflicting models.
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spelling pubmed-29203242010-08-19 Insect Repellents: Modulators of Mosquito Odorant Receptor Activity Bohbot, Jonathan D. Dickens, Joseph C. PLoS One Research Article BACKGROUND: DEET, 2-undecanone (2-U), IR3535 and Picaridin are widely used as insect repellents to prevent interactions between humans and many arthropods including mosquitoes. Their molecular action has only recently been studied, yielding seemingly contradictory theories including odorant-dependent inhibitory and odorant-independent excitatory activities on insect olfactory sensory neurons (OSNs) and odorant receptor proteins (ORs). METHODOLOGY/PRINCIPAL FINDINGS: Here we characterize the action of these repellents on two Aedes aegypti ORs, AaOR2 and AaOR8, individually co-expressed with the common co-receptor AaOR7 in Xenopus oocytes; these ORs are respectively activated by the odors indole (AaOR2) and (R)-(−)-1-octen3-ol (AaOR8), odorants used to locate oviposition sites and host animals. In the absence of odorants, DEET activates AaOR2 but not AaOR8, while 2-U activates AaOR8 but not AaOR2; IR3535 and Picaridin do not activate these ORs. In the presence of odors, DEET strongly inhibits AaOR8 but not AaOR2, while 2-U strongly inhibits AaOR2 but not AaOR8; IR3535 and Picaridin strongly inhibit both ORs. CONCLUSIONS/SIGNIFICANCE: These data demonstrate that repellents can act as olfactory agonists or antagonists, thus modulating OR activity, bringing concordance to conflicting models. Public Library of Science 2010-08-11 /pmc/articles/PMC2920324/ /pubmed/20725637 http://dx.doi.org/10.1371/journal.pone.0012138 Text en This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. https://creativecommons.org/publicdomain/zero/1.0/ This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.
spellingShingle Research Article
Bohbot, Jonathan D.
Dickens, Joseph C.
Insect Repellents: Modulators of Mosquito Odorant Receptor Activity
title Insect Repellents: Modulators of Mosquito Odorant Receptor Activity
title_full Insect Repellents: Modulators of Mosquito Odorant Receptor Activity
title_fullStr Insect Repellents: Modulators of Mosquito Odorant Receptor Activity
title_full_unstemmed Insect Repellents: Modulators of Mosquito Odorant Receptor Activity
title_short Insect Repellents: Modulators of Mosquito Odorant Receptor Activity
title_sort insect repellents: modulators of mosquito odorant receptor activity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920324/
https://www.ncbi.nlm.nih.gov/pubmed/20725637
http://dx.doi.org/10.1371/journal.pone.0012138
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