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RecA-independent single-stranded DNA oligonucleotide-mediated mutagenesis
The expression of Beta, the single-stranded annealing protein (SSAP) of bacteriophage λ in Escherichia coli promotes high levels of oligonucleotide (oligo)-mediated mutagenesis and offers a quick way to create single or multiple base pair insertions, deletions, or substitutions in the bacterial chro...
| Autores principales: | , |
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| Formato: | Texto |
| Lenguaje: | English |
| Publicado: |
Faculty of 1000 Ltd
2010
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920528/ https://www.ncbi.nlm.nih.gov/pubmed/20711416 http://dx.doi.org/10.3410/B2-56 |