Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India

Pyrazinamide (PZA) has been in use for almost 50 years as a first-line drug for short-course chemotherapy against Mycobacterium tuberculosis. In this study, PCR mediated automated DNA sequencing is used to check the prevalence of PZA resistance among treatment failure cases of pulmonary tuberculosis...

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Autores principales: Muthaiah, Muthuraj, Jagadeesan, Sridharan, Ayalusamy, Nisha, Sreenivasan, Manupriya, Prabhu, Sambamurthy Sangamesvara, Muthuraj, Usharani, Senthilkumar, Kamatchiyammal, Veerappan, Saroja
Formato: Texto
Lenguaje:English
Publicado: Molecular Diversity Preservation International (MDPI) 2010
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Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920559/
https://www.ncbi.nlm.nih.gov/pubmed/20717529
http://dx.doi.org/10.3390/ijms11072670
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author Muthaiah, Muthuraj
Jagadeesan, Sridharan
Ayalusamy, Nisha
Sreenivasan, Manupriya
Prabhu, Sambamurthy Sangamesvara
Muthuraj, Usharani
Senthilkumar, Kamatchiyammal
Veerappan, Saroja
author_facet Muthaiah, Muthuraj
Jagadeesan, Sridharan
Ayalusamy, Nisha
Sreenivasan, Manupriya
Prabhu, Sambamurthy Sangamesvara
Muthuraj, Usharani
Senthilkumar, Kamatchiyammal
Veerappan, Saroja
author_sort Muthaiah, Muthuraj
collection PubMed
description Pyrazinamide (PZA) has been in use for almost 50 years as a first-line drug for short-course chemotherapy against Mycobacterium tuberculosis. In this study, PCR mediated automated DNA sequencing is used to check the prevalence of PZA resistance among treatment failure cases of pulmonary tuberculosis. Out of 50 clinical isolates examined, 39 had mutations in the pncA gene that encodes Pyrazinamidase, an enzyme required to activate PZA. Of these, 31 (79.5%) were localized to three regions of pncA. We found two isolates with hitherto unreported mutation at amino acid 26 (Ala→Gly) of pncA.
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spelling pubmed-29205592010-08-17 Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India Muthaiah, Muthuraj Jagadeesan, Sridharan Ayalusamy, Nisha Sreenivasan, Manupriya Prabhu, Sambamurthy Sangamesvara Muthuraj, Usharani Senthilkumar, Kamatchiyammal Veerappan, Saroja Int J Mol Sci Article Pyrazinamide (PZA) has been in use for almost 50 years as a first-line drug for short-course chemotherapy against Mycobacterium tuberculosis. In this study, PCR mediated automated DNA sequencing is used to check the prevalence of PZA resistance among treatment failure cases of pulmonary tuberculosis. Out of 50 clinical isolates examined, 39 had mutations in the pncA gene that encodes Pyrazinamidase, an enzyme required to activate PZA. Of these, 31 (79.5%) were localized to three regions of pncA. We found two isolates with hitherto unreported mutation at amino acid 26 (Ala→Gly) of pncA. Molecular Diversity Preservation International (MDPI) 2010-07-07 /pmc/articles/PMC2920559/ /pubmed/20717529 http://dx.doi.org/10.3390/ijms11072670 Text en © 2010 by the authors; licensee Molecular Diversity Preservation International, Basel, Switzerland. http://creativecommons.org/licenses/by/3.0 This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Article
Muthaiah, Muthuraj
Jagadeesan, Sridharan
Ayalusamy, Nisha
Sreenivasan, Manupriya
Prabhu, Sambamurthy Sangamesvara
Muthuraj, Usharani
Senthilkumar, Kamatchiyammal
Veerappan, Saroja
Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India
title Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India
title_full Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India
title_fullStr Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India
title_full_unstemmed Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India
title_short Molecular Epidemiological Study of Pyrazinamide-Resistance in Clinical Isolates of Mycobacterium tuberculosis from South India
title_sort molecular epidemiological study of pyrazinamide-resistance in clinical isolates of mycobacterium tuberculosis from south india
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920559/
https://www.ncbi.nlm.nih.gov/pubmed/20717529
http://dx.doi.org/10.3390/ijms11072670
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