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Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain
Background: Neuropathic pain is characterized by hyperalgesia, allodynia and spontaneous pain. It often occurs as a result of injury to peripheral nerves, dorsal root ganglions (DRG), spinal cord, or brain. Recent studies have suggested that Toll-like receptor 4 (TLR4) might play a role in neuropath...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920570/ https://www.ncbi.nlm.nih.gov/pubmed/20714435 |
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author | Wu, Fei-xiang Bian, Jin-jun Miao, Xue-rong Huang, Sheng-dong Xu, Xue-wu Gong, De-jun Sun, Yu-ming Lu, Zhi-jie Yu, Wei-feng |
author_facet | Wu, Fei-xiang Bian, Jin-jun Miao, Xue-rong Huang, Sheng-dong Xu, Xue-wu Gong, De-jun Sun, Yu-ming Lu, Zhi-jie Yu, Wei-feng |
author_sort | Wu, Fei-xiang |
collection | PubMed |
description | Background: Neuropathic pain is characterized by hyperalgesia, allodynia and spontaneous pain. It often occurs as a result of injury to peripheral nerves, dorsal root ganglions (DRG), spinal cord, or brain. Recent studies have suggested that Toll-like receptor 4 (TLR4) might play a role in neuropathic pain. Methodology/Principal Findings: In this study, we investigated the role of TLR4 in a rat chronic constriction injury (CCI) model and explored the feasibility of treating neuropathic pain by inhibiting TLR4. Our results demonstrated that intrathecal siRNA-mediated suppression of TLR4 attenuated CCI-induced mechanical allodynia and thermal hyperalgesia through inhibiting the activation of NF-κB p65 and production of proinflammatory cytokines (e.g., TNF-α and IL-1β). Conclusions/Significance: These findings suggest that suppression of TLR4 mediated by intrathecally administered siRNA may be a new strategy for the treatment of neuropathic pain. |
format | Text |
id | pubmed-2920570 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-29205702010-08-16 Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain Wu, Fei-xiang Bian, Jin-jun Miao, Xue-rong Huang, Sheng-dong Xu, Xue-wu Gong, De-jun Sun, Yu-ming Lu, Zhi-jie Yu, Wei-feng Int J Med Sci Research Paper Background: Neuropathic pain is characterized by hyperalgesia, allodynia and spontaneous pain. It often occurs as a result of injury to peripheral nerves, dorsal root ganglions (DRG), spinal cord, or brain. Recent studies have suggested that Toll-like receptor 4 (TLR4) might play a role in neuropathic pain. Methodology/Principal Findings: In this study, we investigated the role of TLR4 in a rat chronic constriction injury (CCI) model and explored the feasibility of treating neuropathic pain by inhibiting TLR4. Our results demonstrated that intrathecal siRNA-mediated suppression of TLR4 attenuated CCI-induced mechanical allodynia and thermal hyperalgesia through inhibiting the activation of NF-κB p65 and production of proinflammatory cytokines (e.g., TNF-α and IL-1β). Conclusions/Significance: These findings suggest that suppression of TLR4 mediated by intrathecally administered siRNA may be a new strategy for the treatment of neuropathic pain. Ivyspring International Publisher 2010-08-02 /pmc/articles/PMC2920570/ /pubmed/20714435 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Wu, Fei-xiang Bian, Jin-jun Miao, Xue-rong Huang, Sheng-dong Xu, Xue-wu Gong, De-jun Sun, Yu-ming Lu, Zhi-jie Yu, Wei-feng Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain |
title | Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain |
title_full | Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain |
title_fullStr | Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain |
title_full_unstemmed | Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain |
title_short | Intrathecal siRNA against Toll-like receptor 4 reduces nociception in a rat model of neuropathic pain |
title_sort | intrathecal sirna against toll-like receptor 4 reduces nociception in a rat model of neuropathic pain |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920570/ https://www.ncbi.nlm.nih.gov/pubmed/20714435 |
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