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Increased invasiveness and aggressiveness in breast epithelia with cytoplasmic p63 expression
Our previous studies revealed that pregnancy associated breast cancer (PABC) had significantly reduced nuclear p63 expression in myoepithelia, while intense cytoplasmic p63 expression in associated epithelia. Our current study assessed these epithelia using immunohistochemistry with a panel of aggre...
Autores principales: | , , , , , |
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Formato: | Texto |
Lenguaje: | English |
Publicado: |
Ivyspring International Publisher
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920576/ https://www.ncbi.nlm.nih.gov/pubmed/20714441 |
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author | Hsiao, Yi-Hsuan Su, Yan A. Tsai, Horng-Der Mason, Jeffrey T. Chou, Ming-Chih Man, Yan-gao |
author_facet | Hsiao, Yi-Hsuan Su, Yan A. Tsai, Horng-Der Mason, Jeffrey T. Chou, Ming-Chih Man, Yan-gao |
author_sort | Hsiao, Yi-Hsuan |
collection | PubMed |
description | Our previous studies revealed that pregnancy associated breast cancer (PABC) had significantly reduced nuclear p63 expression in myoepithelia, while intense cytoplasmic p63 expression in associated epithelia. Our current study assessed these epithelia using immunohistochemistry with a panel of aggressiveness and invasiveness related markers and comparative genomic hybridization (array-CGH) with over 30,000 DNA probes. These epithelia showed several unique alterations, including (1) immunohistochemical and morphological resemblance to invasive cancer, (2) significant gain in copy numbers of DNA coding genes for morphogenesis, angiogenesis, and metastasis, and (3) significant loss in copy numbers of DNA coding genes for tumor suppressors, cell adhesion, and macromolecular complex assembly or intra-cellular trafficking. Detected array-CGH alterations correlated well with in vivo expression of a number of corresponding proteins tested. These findings suggest that aberrant sub-cellular localization of p63 expression in normal or hyperplastic appearing epithelial cells may significant contribute to increased invasiveness and aggressiveness of these cells. |
format | Text |
id | pubmed-2920576 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-29205762010-08-16 Increased invasiveness and aggressiveness in breast epithelia with cytoplasmic p63 expression Hsiao, Yi-Hsuan Su, Yan A. Tsai, Horng-Der Mason, Jeffrey T. Chou, Ming-Chih Man, Yan-gao Int J Biol Sci Research Paper Our previous studies revealed that pregnancy associated breast cancer (PABC) had significantly reduced nuclear p63 expression in myoepithelia, while intense cytoplasmic p63 expression in associated epithelia. Our current study assessed these epithelia using immunohistochemistry with a panel of aggressiveness and invasiveness related markers and comparative genomic hybridization (array-CGH) with over 30,000 DNA probes. These epithelia showed several unique alterations, including (1) immunohistochemical and morphological resemblance to invasive cancer, (2) significant gain in copy numbers of DNA coding genes for morphogenesis, angiogenesis, and metastasis, and (3) significant loss in copy numbers of DNA coding genes for tumor suppressors, cell adhesion, and macromolecular complex assembly or intra-cellular trafficking. Detected array-CGH alterations correlated well with in vivo expression of a number of corresponding proteins tested. These findings suggest that aberrant sub-cellular localization of p63 expression in normal or hyperplastic appearing epithelial cells may significant contribute to increased invasiveness and aggressiveness of these cells. Ivyspring International Publisher 2010-08-08 /pmc/articles/PMC2920576/ /pubmed/20714441 Text en © Ivyspring International Publisher. This is an open-access article distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by-nc-nd/3.0/). Reproduction is permitted for personal, noncommercial use, provided that the article is in whole, unmodified, and properly cited. |
spellingShingle | Research Paper Hsiao, Yi-Hsuan Su, Yan A. Tsai, Horng-Der Mason, Jeffrey T. Chou, Ming-Chih Man, Yan-gao Increased invasiveness and aggressiveness in breast epithelia with cytoplasmic p63 expression |
title | Increased invasiveness and aggressiveness in breast epithelia with cytoplasmic p63 expression |
title_full | Increased invasiveness and aggressiveness in breast epithelia with cytoplasmic p63 expression |
title_fullStr | Increased invasiveness and aggressiveness in breast epithelia with cytoplasmic p63 expression |
title_full_unstemmed | Increased invasiveness and aggressiveness in breast epithelia with cytoplasmic p63 expression |
title_short | Increased invasiveness and aggressiveness in breast epithelia with cytoplasmic p63 expression |
title_sort | increased invasiveness and aggressiveness in breast epithelia with cytoplasmic p63 expression |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920576/ https://www.ncbi.nlm.nih.gov/pubmed/20714441 |
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