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Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina
Gene expression after leaf rust infection was compared in near-isogenic wheat lines differing in the Lr10 leaf rust resistance gene. RNA from susceptible and resistant plants was used for cDNA library construction. In total, 55 008 ESTs were sequenced from the two libraries, then combined and assemb...
Autores principales: | , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Oxford University Press
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920755/ https://www.ncbi.nlm.nih.gov/pubmed/20360266 http://dx.doi.org/10.1093/dnares/dsq009 |
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author | Manickavelu, Alagu Kawaura, Kanako Oishi, Kazuko Shin-I, Tadasu Kohara, Yuji Yahiaoui, Nabila Keller, Beat Suzuki, Ayako Yano, Kentaro Ogihara, Yasunari |
author_facet | Manickavelu, Alagu Kawaura, Kanako Oishi, Kazuko Shin-I, Tadasu Kohara, Yuji Yahiaoui, Nabila Keller, Beat Suzuki, Ayako Yano, Kentaro Ogihara, Yasunari |
author_sort | Manickavelu, Alagu |
collection | PubMed |
description | Gene expression after leaf rust infection was compared in near-isogenic wheat lines differing in the Lr10 leaf rust resistance gene. RNA from susceptible and resistant plants was used for cDNA library construction. In total, 55 008 ESTs were sequenced from the two libraries, then combined and assembled into 14 268 unigenes for further analysis. Of these ESTs, 89% encoded proteins similar to (E value of ≤10(−5)) characterized or annotated proteins from the NCBI non-redundant database representing diverse molecular functions, cellular localization and biological processes based on gene ontology classification. Further, the unigenes were classified into susceptible and resistant classes based on the EST members assembled from the respective libraries. Several genes from the resistant sample (14-3-3 protein, wali5 protein, actin-depolymerization factor and ADP-ribosylation factor) and the susceptible sample (brown plant hopper resistance protein, caffeic acid O-methyltransferase, pathogenesis-related protein and senescence-associated protein) were selected and their differential expression in the resistant and susceptible samples collected at different time points after leaf rust infection was confirmed by RT–PCR analysis. The molecular pathogenicity of leaf rust in wheat was studied and the EST data generated made a foundation for future studies. |
format | Text |
id | pubmed-2920755 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-29207552010-08-12 Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina Manickavelu, Alagu Kawaura, Kanako Oishi, Kazuko Shin-I, Tadasu Kohara, Yuji Yahiaoui, Nabila Keller, Beat Suzuki, Ayako Yano, Kentaro Ogihara, Yasunari DNA Res Full Papers Gene expression after leaf rust infection was compared in near-isogenic wheat lines differing in the Lr10 leaf rust resistance gene. RNA from susceptible and resistant plants was used for cDNA library construction. In total, 55 008 ESTs were sequenced from the two libraries, then combined and assembled into 14 268 unigenes for further analysis. Of these ESTs, 89% encoded proteins similar to (E value of ≤10(−5)) characterized or annotated proteins from the NCBI non-redundant database representing diverse molecular functions, cellular localization and biological processes based on gene ontology classification. Further, the unigenes were classified into susceptible and resistant classes based on the EST members assembled from the respective libraries. Several genes from the resistant sample (14-3-3 protein, wali5 protein, actin-depolymerization factor and ADP-ribosylation factor) and the susceptible sample (brown plant hopper resistance protein, caffeic acid O-methyltransferase, pathogenesis-related protein and senescence-associated protein) were selected and their differential expression in the resistant and susceptible samples collected at different time points after leaf rust infection was confirmed by RT–PCR analysis. The molecular pathogenicity of leaf rust in wheat was studied and the EST data generated made a foundation for future studies. Oxford University Press 2010-08 2010-04-01 /pmc/articles/PMC2920755/ /pubmed/20360266 http://dx.doi.org/10.1093/dnares/dsq009 Text en © The Author 2010. Published by Oxford University Press on behalf of Kazusa DNA Research Institute. http://creativecommons.org/licenses/by-nc/2.5/ This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Full Papers Manickavelu, Alagu Kawaura, Kanako Oishi, Kazuko Shin-I, Tadasu Kohara, Yuji Yahiaoui, Nabila Keller, Beat Suzuki, Ayako Yano, Kentaro Ogihara, Yasunari Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina |
title | Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina |
title_full | Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina |
title_fullStr | Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina |
title_full_unstemmed | Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina |
title_short | Comparative Gene Expression Analysis of Susceptible and Resistant Near-Isogenic Lines in Common Wheat Infected by Puccinia triticina |
title_sort | comparative gene expression analysis of susceptible and resistant near-isogenic lines in common wheat infected by puccinia triticina |
topic | Full Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2920755/ https://www.ncbi.nlm.nih.gov/pubmed/20360266 http://dx.doi.org/10.1093/dnares/dsq009 |
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