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The Closely Related RNA helicases, UAP56 and URH49, Preferentially Form Distinct mRNA Export Machineries and Coordinately Regulate Mitotic Progression

Nuclear export of mRNA is an essential process for eukaryotic gene expression. The TREX complex couples gene expression from transcription and splicing to mRNA export. Sub2, a core component of the TREX complex in yeast, has diversified in humans to two closely related RNA helicases, UAP56 and URH49...

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Autores principales: Yamazaki, Tomohiro, Fujiwara, Naoko, Yukinaga, Hiroko, Ebisuya, Miki, Shiki, Takuya, Kurihara, Tomoya, Kioka, Noriyuki, Kambe, Taiho, Nagao, Masaya, Nishida, Eisuke, Masuda, Seiji
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921121/
https://www.ncbi.nlm.nih.gov/pubmed/20573985
http://dx.doi.org/10.1091/mbc.E09-10-0913
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author Yamazaki, Tomohiro
Fujiwara, Naoko
Yukinaga, Hiroko
Ebisuya, Miki
Shiki, Takuya
Kurihara, Tomoya
Kioka, Noriyuki
Kambe, Taiho
Nagao, Masaya
Nishida, Eisuke
Masuda, Seiji
author_facet Yamazaki, Tomohiro
Fujiwara, Naoko
Yukinaga, Hiroko
Ebisuya, Miki
Shiki, Takuya
Kurihara, Tomoya
Kioka, Noriyuki
Kambe, Taiho
Nagao, Masaya
Nishida, Eisuke
Masuda, Seiji
author_sort Yamazaki, Tomohiro
collection PubMed
description Nuclear export of mRNA is an essential process for eukaryotic gene expression. The TREX complex couples gene expression from transcription and splicing to mRNA export. Sub2, a core component of the TREX complex in yeast, has diversified in humans to two closely related RNA helicases, UAP56 and URH49. Here, we show that URH49 forms a novel URH49–CIP29 complex, termed the AREX (alternative mRNA export) complex, whereas UAP56 forms the human TREX complex. The mRNAs regulated by these helicases are different at the genome-wide level. The two sets of target mRNAs contain distinct subsets of key mitotic regulators. Consistent with their target mRNAs, depletion of UAP56 causes mitotic delay and sister chromatid cohesion defects, whereas depletion of URH49 causes chromosome arm resolution defects and failure of cytokinesis. In addition, depletion of the other human TREX components or CIP29 causes mitotic defects similar to those observed in UAP56- or URH49-depleted cells, respectively. Taken together, the two closely related RNA helicases have evolved to form distinct mRNA export machineries, which regulate mitosis at different steps.
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spelling pubmed-29211212010-10-30 The Closely Related RNA helicases, UAP56 and URH49, Preferentially Form Distinct mRNA Export Machineries and Coordinately Regulate Mitotic Progression Yamazaki, Tomohiro Fujiwara, Naoko Yukinaga, Hiroko Ebisuya, Miki Shiki, Takuya Kurihara, Tomoya Kioka, Noriyuki Kambe, Taiho Nagao, Masaya Nishida, Eisuke Masuda, Seiji Mol Biol Cell Articles Nuclear export of mRNA is an essential process for eukaryotic gene expression. The TREX complex couples gene expression from transcription and splicing to mRNA export. Sub2, a core component of the TREX complex in yeast, has diversified in humans to two closely related RNA helicases, UAP56 and URH49. Here, we show that URH49 forms a novel URH49–CIP29 complex, termed the AREX (alternative mRNA export) complex, whereas UAP56 forms the human TREX complex. The mRNAs regulated by these helicases are different at the genome-wide level. The two sets of target mRNAs contain distinct subsets of key mitotic regulators. Consistent with their target mRNAs, depletion of UAP56 causes mitotic delay and sister chromatid cohesion defects, whereas depletion of URH49 causes chromosome arm resolution defects and failure of cytokinesis. In addition, depletion of the other human TREX components or CIP29 causes mitotic defects similar to those observed in UAP56- or URH49-depleted cells, respectively. Taken together, the two closely related RNA helicases have evolved to form distinct mRNA export machineries, which regulate mitosis at different steps. The American Society for Cell Biology 2010-08-15 /pmc/articles/PMC2921121/ /pubmed/20573985 http://dx.doi.org/10.1091/mbc.E09-10-0913 Text en © 2010 by The American Society for Cell Biology
spellingShingle Articles
Yamazaki, Tomohiro
Fujiwara, Naoko
Yukinaga, Hiroko
Ebisuya, Miki
Shiki, Takuya
Kurihara, Tomoya
Kioka, Noriyuki
Kambe, Taiho
Nagao, Masaya
Nishida, Eisuke
Masuda, Seiji
The Closely Related RNA helicases, UAP56 and URH49, Preferentially Form Distinct mRNA Export Machineries and Coordinately Regulate Mitotic Progression
title The Closely Related RNA helicases, UAP56 and URH49, Preferentially Form Distinct mRNA Export Machineries and Coordinately Regulate Mitotic Progression
title_full The Closely Related RNA helicases, UAP56 and URH49, Preferentially Form Distinct mRNA Export Machineries and Coordinately Regulate Mitotic Progression
title_fullStr The Closely Related RNA helicases, UAP56 and URH49, Preferentially Form Distinct mRNA Export Machineries and Coordinately Regulate Mitotic Progression
title_full_unstemmed The Closely Related RNA helicases, UAP56 and URH49, Preferentially Form Distinct mRNA Export Machineries and Coordinately Regulate Mitotic Progression
title_short The Closely Related RNA helicases, UAP56 and URH49, Preferentially Form Distinct mRNA Export Machineries and Coordinately Regulate Mitotic Progression
title_sort closely related rna helicases, uap56 and urh49, preferentially form distinct mrna export machineries and coordinately regulate mitotic progression
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921121/
https://www.ncbi.nlm.nih.gov/pubmed/20573985
http://dx.doi.org/10.1091/mbc.E09-10-0913
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