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Quantitative Analysis of the Mechanism of Endocytic Actin Patch Assembly and Disassembly in Fission Yeast

We used quantitative confocal microscopy to measure the numbers of 16 proteins tagged with fluorescent proteins during assembly and disassembly of endocytic actin patches in fission yeast. The peak numbers of each molecule that accumulate in patches varied <30–50% between individual patches. The...

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Detalles Bibliográficos
Autores principales: Sirotkin, Vladimir, Berro, Julien, Macmillan, Keely, Zhao, Lindsey, Pollard, Thomas D.
Formato: Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921122/
https://www.ncbi.nlm.nih.gov/pubmed/20587778
http://dx.doi.org/10.1091/mbc.E10-02-0157