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Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity

BACKGROUND: A proteolytic imbalance has been implicated in the development of “classical” chronic lung disease of prematurity (CLD). However, in “new” CLD this pattern has changed. This study examines the longitudinal relationship between neutrophil proteinases and their inhibitors in ventilated pre...

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Autores principales: Davies, Philip L, Spiller, O Brad, Beeton, Michael L, Maxwell, Nicola C, Remold-O'Donnell, Eileen, Kotecha, Sailesh
Formato: Texto
Lenguaje:English
Publicado: BMJ Group 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921268/
https://www.ncbi.nlm.nih.gov/pubmed/20335295
http://dx.doi.org/10.1136/thx.2009.116061
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author Davies, Philip L
Spiller, O Brad
Beeton, Michael L
Maxwell, Nicola C
Remold-O'Donnell, Eileen
Kotecha, Sailesh
author_facet Davies, Philip L
Spiller, O Brad
Beeton, Michael L
Maxwell, Nicola C
Remold-O'Donnell, Eileen
Kotecha, Sailesh
author_sort Davies, Philip L
collection PubMed
description BACKGROUND: A proteolytic imbalance has been implicated in the development of “classical” chronic lung disease of prematurity (CLD). However, in “new” CLD this pattern has changed. This study examines the longitudinal relationship between neutrophil proteinases and their inhibitors in ventilated preterm infants and their relationship to microbial colonisation. METHODS: Serial bronchoalveolar lavage fluid was obtained from ventilated newborn preterm infants. Neutrophil elastase (NE) activity, cell counts, metalloproteinase (MMP)-9, MMP-9/TIMP-1 complex, SerpinB1 concentration and percentage of SerpinB1 and α(1)-antitrypsin (AAT) in complex with elastase were measured. The presence of microbial genes was examined using PCR for 16S rRNA genes. RESULTS: Statistically more infants who developed CLD had NE activity in at least one sample (10/20) compared with infants with resolved respiratory distress syndrome (RDS) (2/17). However, NE activity was present in a minority of samples, occurring as episodic peaks. Peak levels of MMP-9, MMP-9/TIMP-1 complex, percentage of AAT and SerpinB1 in complex and cell counts were all statistically greater in infants developing CLD than in infants with resolved RDS. Peak values frequently occurred as episodic spikes and strong temporal relationships were noted between all markers. The peak values for all variables were significantly correlated to each other. The presence of bacterial 16S rRNA genes was associated with the development of CLD and with elevated elastase and MMP-9. CONCLUSION: NE activity and MMP-9 appear to be important in the development of “new” CLD with both proteinase and inhibitor concentrations increasing episodically, possibly in response to postnatal infection.
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spelling pubmed-29212682010-08-17 Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity Davies, Philip L Spiller, O Brad Beeton, Michael L Maxwell, Nicola C Remold-O'Donnell, Eileen Kotecha, Sailesh Thorax Paediatric Lung Disease BACKGROUND: A proteolytic imbalance has been implicated in the development of “classical” chronic lung disease of prematurity (CLD). However, in “new” CLD this pattern has changed. This study examines the longitudinal relationship between neutrophil proteinases and their inhibitors in ventilated preterm infants and their relationship to microbial colonisation. METHODS: Serial bronchoalveolar lavage fluid was obtained from ventilated newborn preterm infants. Neutrophil elastase (NE) activity, cell counts, metalloproteinase (MMP)-9, MMP-9/TIMP-1 complex, SerpinB1 concentration and percentage of SerpinB1 and α(1)-antitrypsin (AAT) in complex with elastase were measured. The presence of microbial genes was examined using PCR for 16S rRNA genes. RESULTS: Statistically more infants who developed CLD had NE activity in at least one sample (10/20) compared with infants with resolved respiratory distress syndrome (RDS) (2/17). However, NE activity was present in a minority of samples, occurring as episodic peaks. Peak levels of MMP-9, MMP-9/TIMP-1 complex, percentage of AAT and SerpinB1 in complex and cell counts were all statistically greater in infants developing CLD than in infants with resolved RDS. Peak values frequently occurred as episodic spikes and strong temporal relationships were noted between all markers. The peak values for all variables were significantly correlated to each other. The presence of bacterial 16S rRNA genes was associated with the development of CLD and with elevated elastase and MMP-9. CONCLUSION: NE activity and MMP-9 appear to be important in the development of “new” CLD with both proteinase and inhibitor concentrations increasing episodically, possibly in response to postnatal infection. BMJ Group 2010-02-27 2010-03 /pmc/articles/PMC2921268/ /pubmed/20335295 http://dx.doi.org/10.1136/thx.2009.116061 Text en © 2010, Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-commercial License, which permits use, distribution, and reproduction in any medium, provided the original work is properly cited, the use is non commercial and is otherwise in compliance with the license. See: http://creativecommons.org/licenses/by-nc/2.0/ and http://creativecommons.org/licenses/by-nc/2.0/legalcode.
spellingShingle Paediatric Lung Disease
Davies, Philip L
Spiller, O Brad
Beeton, Michael L
Maxwell, Nicola C
Remold-O'Donnell, Eileen
Kotecha, Sailesh
Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity
title Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity
title_full Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity
title_fullStr Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity
title_full_unstemmed Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity
title_short Relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity
title_sort relationship of proteinases and proteinase inhibitors with microbial presence in chronic lung disease of prematurity
topic Paediatric Lung Disease
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921268/
https://www.ncbi.nlm.nih.gov/pubmed/20335295
http://dx.doi.org/10.1136/thx.2009.116061
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