Sampling and pyrosequencing methods for characterizing bacterial communities in the human gut using 16S sequence tags

Intense interest centers on the role of the human gut microbiome in health and disease, but optimal methods for analysis are still under development. Here we present a study of methods for surveying bacterial communities in human feces using 454/Roche pyrosequencing of 16S rRNA gene tags. We analyze...

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Detalles Bibliográficos
Autores principales: Wu, Gary D, Lewis, James D, Hoffmann, Christian, Chen, Ying-Yu, Knight, Rob, Bittinger, Kyle, Hwang, Jennifer, Chen, Jun, Berkowsky, Ronald, Nessel, Lisa, Li, Hongzhe, Bushman, Frederic D
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Methodology Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921404/
https://www.ncbi.nlm.nih.gov/pubmed/20673359
http://dx.doi.org/10.1186/1471-2180-10-206

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