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Syndecan-3 and Notch cooperate in regulating adult myogenesis

Skeletal muscle postnatal growth and repair depend on satellite cells and are regulated by molecular signals within the satellite cell niche. We investigated the molecular and cellular events that lead to altered myogenesis upon genetic ablation of Syndecan-3, a component of the satellite cell niche...

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Detalles Bibliográficos
Autores principales: Pisconti, Addolorata, Cornelison, D.D.W., Olguín, Hugo C., Antwine, Tiffany L., Olwin, Bradley B.
Formato: Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2922652/
https://www.ncbi.nlm.nih.gov/pubmed/20696709
http://dx.doi.org/10.1083/jcb.201003081
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author Pisconti, Addolorata
Cornelison, D.D.W.
Olguín, Hugo C.
Antwine, Tiffany L.
Olwin, Bradley B.
author_facet Pisconti, Addolorata
Cornelison, D.D.W.
Olguín, Hugo C.
Antwine, Tiffany L.
Olwin, Bradley B.
author_sort Pisconti, Addolorata
collection PubMed
description Skeletal muscle postnatal growth and repair depend on satellite cells and are regulated by molecular signals within the satellite cell niche. We investigated the molecular and cellular events that lead to altered myogenesis upon genetic ablation of Syndecan-3, a component of the satellite cell niche. In the absence of Syndecan-3, satellite cells stall in S phase, leading to reduced proliferation, increased cell death, delayed onset of differentiation, and markedly reduced numbers of Pax7(+) satellite cells accompanied by myofiber hypertrophy and an increased number of centrally nucleated myofibers. We show that the aberrant cell cycle and impaired self-renewal of explanted Syndecan-3–null satellite cells are rescued by ectopic expression of the constitutively active Notch intracellular domain. Furthermore, we show that Syndecan-3 interacts with Notch and is required for Notch processing by ADAM17/tumor necrosis factor-α–converting enzyme (TACE) and signal transduction. Together, our data support the conclusion that Syndecan-3 and Notch cooperate in regulating homeostasis of the satellite cell population and myofiber size.
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spelling pubmed-29226522011-02-09 Syndecan-3 and Notch cooperate in regulating adult myogenesis Pisconti, Addolorata Cornelison, D.D.W. Olguín, Hugo C. Antwine, Tiffany L. Olwin, Bradley B. J Cell Biol Research Articles Skeletal muscle postnatal growth and repair depend on satellite cells and are regulated by molecular signals within the satellite cell niche. We investigated the molecular and cellular events that lead to altered myogenesis upon genetic ablation of Syndecan-3, a component of the satellite cell niche. In the absence of Syndecan-3, satellite cells stall in S phase, leading to reduced proliferation, increased cell death, delayed onset of differentiation, and markedly reduced numbers of Pax7(+) satellite cells accompanied by myofiber hypertrophy and an increased number of centrally nucleated myofibers. We show that the aberrant cell cycle and impaired self-renewal of explanted Syndecan-3–null satellite cells are rescued by ectopic expression of the constitutively active Notch intracellular domain. Furthermore, we show that Syndecan-3 interacts with Notch and is required for Notch processing by ADAM17/tumor necrosis factor-α–converting enzyme (TACE) and signal transduction. Together, our data support the conclusion that Syndecan-3 and Notch cooperate in regulating homeostasis of the satellite cell population and myofiber size. The Rockefeller University Press 2010-08-09 /pmc/articles/PMC2922652/ /pubmed/20696709 http://dx.doi.org/10.1083/jcb.201003081 Text en © 2010 Pisconti et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Research Articles
Pisconti, Addolorata
Cornelison, D.D.W.
Olguín, Hugo C.
Antwine, Tiffany L.
Olwin, Bradley B.
Syndecan-3 and Notch cooperate in regulating adult myogenesis
title Syndecan-3 and Notch cooperate in regulating adult myogenesis
title_full Syndecan-3 and Notch cooperate in regulating adult myogenesis
title_fullStr Syndecan-3 and Notch cooperate in regulating adult myogenesis
title_full_unstemmed Syndecan-3 and Notch cooperate in regulating adult myogenesis
title_short Syndecan-3 and Notch cooperate in regulating adult myogenesis
title_sort syndecan-3 and notch cooperate in regulating adult myogenesis
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2922652/
https://www.ncbi.nlm.nih.gov/pubmed/20696709
http://dx.doi.org/10.1083/jcb.201003081
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