CD169(+) MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES

Invariant NKT (iNKT) cells are involved in host defence against microbial infections. While it is known that iNKT cells recognize glycolipids presented by CD1d, how and where they encounter antigen in vivo remains unclear. We used multi-photon microscopy to visualize the dynamics and activation of i...

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Autores principales: Barral, Patricia, Polzella, Paolo, Bruckbauer, Andreas, van Rooijen, Nico, Besra, Gurdyal S., Cerundolo, Vincenzo, Batista, Facundo D.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923071/
https://www.ncbi.nlm.nih.gov/pubmed/20228797
http://dx.doi.org/10.1038/ni.1853
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author Barral, Patricia
Polzella, Paolo
Bruckbauer, Andreas
van Rooijen, Nico
Besra, Gurdyal S.
Cerundolo, Vincenzo
Batista, Facundo D.
author_facet Barral, Patricia
Polzella, Paolo
Bruckbauer, Andreas
van Rooijen, Nico
Besra, Gurdyal S.
Cerundolo, Vincenzo
Batista, Facundo D.
author_sort Barral, Patricia
collection PubMed
description Invariant NKT (iNKT) cells are involved in host defence against microbial infections. While it is known that iNKT cells recognize glycolipids presented by CD1d, how and where they encounter antigen in vivo remains unclear. We used multi-photon microscopy to visualize the dynamics and activation of iNKT cells in lymph nodes. Following antigen administration, iNKT cells become confined in a CD1d-dependent manner in close proximity to subcapsular sinus CD169(+) macrophages. These macrophages retain, internalize and present lipid antigen, and are required for iNKT cell activation, cytokine production and expansion. Thus, CD169(+) macrophages can act as bona fide antigen presenting cells controlling early iNKT cell activation and favouring fast initiation of immune responses.
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spelling pubmed-29230712010-10-01 CD169(+) MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES Barral, Patricia Polzella, Paolo Bruckbauer, Andreas van Rooijen, Nico Besra, Gurdyal S. Cerundolo, Vincenzo Batista, Facundo D. Nat Immunol Article Invariant NKT (iNKT) cells are involved in host defence against microbial infections. While it is known that iNKT cells recognize glycolipids presented by CD1d, how and where they encounter antigen in vivo remains unclear. We used multi-photon microscopy to visualize the dynamics and activation of iNKT cells in lymph nodes. Following antigen administration, iNKT cells become confined in a CD1d-dependent manner in close proximity to subcapsular sinus CD169(+) macrophages. These macrophages retain, internalize and present lipid antigen, and are required for iNKT cell activation, cytokine production and expansion. Thus, CD169(+) macrophages can act as bona fide antigen presenting cells controlling early iNKT cell activation and favouring fast initiation of immune responses. 2010-03-14 2010-04 /pmc/articles/PMC2923071/ /pubmed/20228797 http://dx.doi.org/10.1038/ni.1853 Text en Users may view, print, copy, download and text and data- mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use: http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Barral, Patricia
Polzella, Paolo
Bruckbauer, Andreas
van Rooijen, Nico
Besra, Gurdyal S.
Cerundolo, Vincenzo
Batista, Facundo D.
CD169(+) MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES
title CD169(+) MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES
title_full CD169(+) MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES
title_fullStr CD169(+) MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES
title_full_unstemmed CD169(+) MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES
title_short CD169(+) MACROPHAGES PRESENT LIPID ANTIGENS TO MEDIATE EARLY ACTIVATION OF INVARIANT NKT CELLS IN LYMPH NODES
title_sort cd169(+) macrophages present lipid antigens to mediate early activation of invariant nkt cells in lymph nodes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923071/
https://www.ncbi.nlm.nih.gov/pubmed/20228797
http://dx.doi.org/10.1038/ni.1853
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