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bFGF Regulates PI3-Kinase-Rac1-JNK Pathway and Promotes Fibroblast Migration in Wound Healing
Fibroblast proliferation and migration play important roles in wound healing. bFGF is known to promote both fibroblast proliferation and migration during the process of wound healing. However, the signal transduction of bFGF-induced fibroblast migration is still unclear, because bFGF can affect both...
Autores principales: | , , , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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Public Library of Science
2010
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923192/ https://www.ncbi.nlm.nih.gov/pubmed/20808927 http://dx.doi.org/10.1371/journal.pone.0012228 |
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author | Kanazawa, Shigeyuki Fujiwara, Toshihiro Matsuzaki, Shinsuke Shingaki, Kenta Taniguchi, Manabu Miyata, Shingo Tohyama, Masaya Sakai, Yasuo Yano, Kenji Hosokawa, Ko Kubo, Tateki |
author_facet | Kanazawa, Shigeyuki Fujiwara, Toshihiro Matsuzaki, Shinsuke Shingaki, Kenta Taniguchi, Manabu Miyata, Shingo Tohyama, Masaya Sakai, Yasuo Yano, Kenji Hosokawa, Ko Kubo, Tateki |
author_sort | Kanazawa, Shigeyuki |
collection | PubMed |
description | Fibroblast proliferation and migration play important roles in wound healing. bFGF is known to promote both fibroblast proliferation and migration during the process of wound healing. However, the signal transduction of bFGF-induced fibroblast migration is still unclear, because bFGF can affect both proliferation and migration. Herein, we investigated the effect of bFGF on fibroblast migration regardless of its effect on fibroblast proliferation. We noticed involvement of the small GTPases of the Rho family, PI3-kinase, and JNK. bFGF activated RhoA, Rac1, PI3-kinase, and JNK in cultured fibroblasts. Inhibition of RhoA did not block bFGF-induced fibroblast migration, whereas inhibition of Rac1, PI3-kinase, or JNK blocked the fibroblast migration significantly. PI3-kinase-inhibited cells down-regulated the activities of Rac1 and JNK, and Rac1-inhibited cells down-regulated JNK activity, suggesting that PI3-kinase is upstream of Rac1 and that JNK is downstream of Rac1. Thus, we concluded that PI3-kinase, Rac1, and JNK were essential for bFGF-induced fibroblast migration, which is a novel pathway of bFGF-induced cell migration. |
format | Text |
id | pubmed-2923192 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-29231922010-08-31 bFGF Regulates PI3-Kinase-Rac1-JNK Pathway and Promotes Fibroblast Migration in Wound Healing Kanazawa, Shigeyuki Fujiwara, Toshihiro Matsuzaki, Shinsuke Shingaki, Kenta Taniguchi, Manabu Miyata, Shingo Tohyama, Masaya Sakai, Yasuo Yano, Kenji Hosokawa, Ko Kubo, Tateki PLoS One Research Article Fibroblast proliferation and migration play important roles in wound healing. bFGF is known to promote both fibroblast proliferation and migration during the process of wound healing. However, the signal transduction of bFGF-induced fibroblast migration is still unclear, because bFGF can affect both proliferation and migration. Herein, we investigated the effect of bFGF on fibroblast migration regardless of its effect on fibroblast proliferation. We noticed involvement of the small GTPases of the Rho family, PI3-kinase, and JNK. bFGF activated RhoA, Rac1, PI3-kinase, and JNK in cultured fibroblasts. Inhibition of RhoA did not block bFGF-induced fibroblast migration, whereas inhibition of Rac1, PI3-kinase, or JNK blocked the fibroblast migration significantly. PI3-kinase-inhibited cells down-regulated the activities of Rac1 and JNK, and Rac1-inhibited cells down-regulated JNK activity, suggesting that PI3-kinase is upstream of Rac1 and that JNK is downstream of Rac1. Thus, we concluded that PI3-kinase, Rac1, and JNK were essential for bFGF-induced fibroblast migration, which is a novel pathway of bFGF-induced cell migration. Public Library of Science 2010-08-17 /pmc/articles/PMC2923192/ /pubmed/20808927 http://dx.doi.org/10.1371/journal.pone.0012228 Text en Kanazawa et al. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Kanazawa, Shigeyuki Fujiwara, Toshihiro Matsuzaki, Shinsuke Shingaki, Kenta Taniguchi, Manabu Miyata, Shingo Tohyama, Masaya Sakai, Yasuo Yano, Kenji Hosokawa, Ko Kubo, Tateki bFGF Regulates PI3-Kinase-Rac1-JNK Pathway and Promotes Fibroblast Migration in Wound Healing |
title | bFGF Regulates PI3-Kinase-Rac1-JNK Pathway and Promotes Fibroblast Migration in Wound Healing |
title_full | bFGF Regulates PI3-Kinase-Rac1-JNK Pathway and Promotes Fibroblast Migration in Wound Healing |
title_fullStr | bFGF Regulates PI3-Kinase-Rac1-JNK Pathway and Promotes Fibroblast Migration in Wound Healing |
title_full_unstemmed | bFGF Regulates PI3-Kinase-Rac1-JNK Pathway and Promotes Fibroblast Migration in Wound Healing |
title_short | bFGF Regulates PI3-Kinase-Rac1-JNK Pathway and Promotes Fibroblast Migration in Wound Healing |
title_sort | bfgf regulates pi3-kinase-rac1-jnk pathway and promotes fibroblast migration in wound healing |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923192/ https://www.ncbi.nlm.nih.gov/pubmed/20808927 http://dx.doi.org/10.1371/journal.pone.0012228 |
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