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Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs
BACKGROUND: Mitochondria are dynamic organelles that move along actin filaments, and serve as calcium stores in plant cells. The positioning and dynamics of mitochondria depend on membrane-cytoskeleton interactions, but it is not clear whether microfilament cytoskeleton has a direct effect on mitoch...
Autores principales: | , , , , , , , , |
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Formato: | Texto |
Lenguaje: | English |
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BioMed Central
2010
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923527/ https://www.ncbi.nlm.nih.gov/pubmed/20334630 http://dx.doi.org/10.1186/1471-2229-10-53 |
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author | Wang, Yuqing Zhu, Yingfang Ling, Yu Zhang, Haiyan Liu, Peng Baluška, František Šamaj, Jozef Lin, Jinxing Wang, Qinli |
author_facet | Wang, Yuqing Zhu, Yingfang Ling, Yu Zhang, Haiyan Liu, Peng Baluška, František Šamaj, Jozef Lin, Jinxing Wang, Qinli |
author_sort | Wang, Yuqing |
collection | PubMed |
description | BACKGROUND: Mitochondria are dynamic organelles that move along actin filaments, and serve as calcium stores in plant cells. The positioning and dynamics of mitochondria depend on membrane-cytoskeleton interactions, but it is not clear whether microfilament cytoskeleton has a direct effect on mitochondrial function and Ca(2+ )storage. Therefore, we designed a series of experiments to clarify the effects of actin filaments on mitochondrial Ca(2+ )storage, cytoplasmic Ca(2+ )concentration ([Ca(2+)](c)), and the interaction between mitochondrial Ca(2+ )and cytoplasmic Ca(2+ )in Arabidopsis root hairs. RESULTS: In this study, we found that treatments with latrunculin B (Lat-B) and jasplakinolide (Jas), which depolymerize and polymerize actin filaments respectively, decreased membrane potential and Ca(2+ )stores in the mitochondria of Arabidopsis root hairs. Simultaneously, these treatments induced an instantaneous increase of cytoplasmic Ca(2+), followed by a continuous decrease. All of these effects were inhibited by pretreatment with cyclosporin A (Cs A), a representative blocker of the mitochondrial permeability transition pore (mPTP). Moreover, we found there was a Ca(2+ )concentration gradient in mitochondria from the tip to the base of the root hair, and this gradient could be disrupted by actin-acting drugs. CONCLUSIONS: Based on these results, we concluded that the disruption of actin filaments caused by Lat-B or Jas promoted irreversible opening of the mPTP, resulting in mitochondrial Ca(2+ )release into the cytoplasm, and consequent changes in [Ca(2+)](c). We suggest that normal polymerization and depolymerization of actin filaments are essential for mitochondrial Ca(2+ )storage in root hairs. |
format | Text |
id | pubmed-2923527 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2010 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-29235272010-08-19 Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs Wang, Yuqing Zhu, Yingfang Ling, Yu Zhang, Haiyan Liu, Peng Baluška, František Šamaj, Jozef Lin, Jinxing Wang, Qinli BMC Plant Biol Research Article BACKGROUND: Mitochondria are dynamic organelles that move along actin filaments, and serve as calcium stores in plant cells. The positioning and dynamics of mitochondria depend on membrane-cytoskeleton interactions, but it is not clear whether microfilament cytoskeleton has a direct effect on mitochondrial function and Ca(2+ )storage. Therefore, we designed a series of experiments to clarify the effects of actin filaments on mitochondrial Ca(2+ )storage, cytoplasmic Ca(2+ )concentration ([Ca(2+)](c)), and the interaction between mitochondrial Ca(2+ )and cytoplasmic Ca(2+ )in Arabidopsis root hairs. RESULTS: In this study, we found that treatments with latrunculin B (Lat-B) and jasplakinolide (Jas), which depolymerize and polymerize actin filaments respectively, decreased membrane potential and Ca(2+ )stores in the mitochondria of Arabidopsis root hairs. Simultaneously, these treatments induced an instantaneous increase of cytoplasmic Ca(2+), followed by a continuous decrease. All of these effects were inhibited by pretreatment with cyclosporin A (Cs A), a representative blocker of the mitochondrial permeability transition pore (mPTP). Moreover, we found there was a Ca(2+ )concentration gradient in mitochondria from the tip to the base of the root hair, and this gradient could be disrupted by actin-acting drugs. CONCLUSIONS: Based on these results, we concluded that the disruption of actin filaments caused by Lat-B or Jas promoted irreversible opening of the mPTP, resulting in mitochondrial Ca(2+ )release into the cytoplasm, and consequent changes in [Ca(2+)](c). We suggest that normal polymerization and depolymerization of actin filaments are essential for mitochondrial Ca(2+ )storage in root hairs. BioMed Central 2010-03-24 /pmc/articles/PMC2923527/ /pubmed/20334630 http://dx.doi.org/10.1186/1471-2229-10-53 Text en Copyright ©2010 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Wang, Yuqing Zhu, Yingfang Ling, Yu Zhang, Haiyan Liu, Peng Baluška, František Šamaj, Jozef Lin, Jinxing Wang, Qinli Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs |
title | Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs |
title_full | Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs |
title_fullStr | Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs |
title_full_unstemmed | Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs |
title_short | Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs |
title_sort | disruption of actin filaments induces mitochondrial ca(2+ )release to the cytoplasm and [ca(2+)](c )changes in arabidopsis root hairs |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923527/ https://www.ncbi.nlm.nih.gov/pubmed/20334630 http://dx.doi.org/10.1186/1471-2229-10-53 |
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