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Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs

BACKGROUND: Mitochondria are dynamic organelles that move along actin filaments, and serve as calcium stores in plant cells. The positioning and dynamics of mitochondria depend on membrane-cytoskeleton interactions, but it is not clear whether microfilament cytoskeleton has a direct effect on mitoch...

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Autores principales: Wang, Yuqing, Zhu, Yingfang, Ling, Yu, Zhang, Haiyan, Liu, Peng, Baluška, František, Šamaj, Jozef, Lin, Jinxing, Wang, Qinli
Formato: Texto
Lenguaje:English
Publicado: BioMed Central 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923527/
https://www.ncbi.nlm.nih.gov/pubmed/20334630
http://dx.doi.org/10.1186/1471-2229-10-53
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author Wang, Yuqing
Zhu, Yingfang
Ling, Yu
Zhang, Haiyan
Liu, Peng
Baluška, František
Šamaj, Jozef
Lin, Jinxing
Wang, Qinli
author_facet Wang, Yuqing
Zhu, Yingfang
Ling, Yu
Zhang, Haiyan
Liu, Peng
Baluška, František
Šamaj, Jozef
Lin, Jinxing
Wang, Qinli
author_sort Wang, Yuqing
collection PubMed
description BACKGROUND: Mitochondria are dynamic organelles that move along actin filaments, and serve as calcium stores in plant cells. The positioning and dynamics of mitochondria depend on membrane-cytoskeleton interactions, but it is not clear whether microfilament cytoskeleton has a direct effect on mitochondrial function and Ca(2+ )storage. Therefore, we designed a series of experiments to clarify the effects of actin filaments on mitochondrial Ca(2+ )storage, cytoplasmic Ca(2+ )concentration ([Ca(2+)](c)), and the interaction between mitochondrial Ca(2+ )and cytoplasmic Ca(2+ )in Arabidopsis root hairs. RESULTS: In this study, we found that treatments with latrunculin B (Lat-B) and jasplakinolide (Jas), which depolymerize and polymerize actin filaments respectively, decreased membrane potential and Ca(2+ )stores in the mitochondria of Arabidopsis root hairs. Simultaneously, these treatments induced an instantaneous increase of cytoplasmic Ca(2+), followed by a continuous decrease. All of these effects were inhibited by pretreatment with cyclosporin A (Cs A), a representative blocker of the mitochondrial permeability transition pore (mPTP). Moreover, we found there was a Ca(2+ )concentration gradient in mitochondria from the tip to the base of the root hair, and this gradient could be disrupted by actin-acting drugs. CONCLUSIONS: Based on these results, we concluded that the disruption of actin filaments caused by Lat-B or Jas promoted irreversible opening of the mPTP, resulting in mitochondrial Ca(2+ )release into the cytoplasm, and consequent changes in [Ca(2+)](c). We suggest that normal polymerization and depolymerization of actin filaments are essential for mitochondrial Ca(2+ )storage in root hairs.
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spelling pubmed-29235272010-08-19 Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs Wang, Yuqing Zhu, Yingfang Ling, Yu Zhang, Haiyan Liu, Peng Baluška, František Šamaj, Jozef Lin, Jinxing Wang, Qinli BMC Plant Biol Research Article BACKGROUND: Mitochondria are dynamic organelles that move along actin filaments, and serve as calcium stores in plant cells. The positioning and dynamics of mitochondria depend on membrane-cytoskeleton interactions, but it is not clear whether microfilament cytoskeleton has a direct effect on mitochondrial function and Ca(2+ )storage. Therefore, we designed a series of experiments to clarify the effects of actin filaments on mitochondrial Ca(2+ )storage, cytoplasmic Ca(2+ )concentration ([Ca(2+)](c)), and the interaction between mitochondrial Ca(2+ )and cytoplasmic Ca(2+ )in Arabidopsis root hairs. RESULTS: In this study, we found that treatments with latrunculin B (Lat-B) and jasplakinolide (Jas), which depolymerize and polymerize actin filaments respectively, decreased membrane potential and Ca(2+ )stores in the mitochondria of Arabidopsis root hairs. Simultaneously, these treatments induced an instantaneous increase of cytoplasmic Ca(2+), followed by a continuous decrease. All of these effects were inhibited by pretreatment with cyclosporin A (Cs A), a representative blocker of the mitochondrial permeability transition pore (mPTP). Moreover, we found there was a Ca(2+ )concentration gradient in mitochondria from the tip to the base of the root hair, and this gradient could be disrupted by actin-acting drugs. CONCLUSIONS: Based on these results, we concluded that the disruption of actin filaments caused by Lat-B or Jas promoted irreversible opening of the mPTP, resulting in mitochondrial Ca(2+ )release into the cytoplasm, and consequent changes in [Ca(2+)](c). We suggest that normal polymerization and depolymerization of actin filaments are essential for mitochondrial Ca(2+ )storage in root hairs. BioMed Central 2010-03-24 /pmc/articles/PMC2923527/ /pubmed/20334630 http://dx.doi.org/10.1186/1471-2229-10-53 Text en Copyright ©2010 Wang et al; licensee BioMed Central Ltd. http://creativecommons.org/licenses/by/2.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wang, Yuqing
Zhu, Yingfang
Ling, Yu
Zhang, Haiyan
Liu, Peng
Baluška, František
Šamaj, Jozef
Lin, Jinxing
Wang, Qinli
Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs
title Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs
title_full Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs
title_fullStr Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs
title_full_unstemmed Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs
title_short Disruption of actin filaments induces mitochondrial Ca(2+ )release to the cytoplasm and [Ca(2+)](c )changes in Arabidopsis root hairs
title_sort disruption of actin filaments induces mitochondrial ca(2+ )release to the cytoplasm and [ca(2+)](c )changes in arabidopsis root hairs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2923527/
https://www.ncbi.nlm.nih.gov/pubmed/20334630
http://dx.doi.org/10.1186/1471-2229-10-53
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