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Mucosal Delivery of Human Papillomavirus Pseudovirus-Encapsidated Plasmids Improves Potency of DNA Vaccination

Mucosal immunization may be important for protection against pathogens whose transmission and pathogenesis target mucosal tissue. The capsid proteins of human papillomavirus (HPV) confer tropism for basal epithelium and can encapsidate DNA during self-assembly to form pseudovirions (PsV). Therefore,...

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Autores principales: Graham, Barney S., Kines, Rhonda, Corbett, Kizzmekia S., Nicewonger, John, Johnson, Teresa R., Chen, Man, LaVigne, Daaimah, Roberts, Jeffrey N., Cuburu, Nicolas, Schiller, John T., Buck, Christopher B.
Formato: Texto
Lenguaje:English
Publicado: 2010
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924464/
https://www.ncbi.nlm.nih.gov/pubmed/20555315
http://dx.doi.org/10.1038/mi.2010.31
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author Graham, Barney S.
Kines, Rhonda
Corbett, Kizzmekia S.
Nicewonger, John
Johnson, Teresa R.
Chen, Man
LaVigne, Daaimah
Roberts, Jeffrey N.
Cuburu, Nicolas
Schiller, John T.
Buck, Christopher B.
author_facet Graham, Barney S.
Kines, Rhonda
Corbett, Kizzmekia S.
Nicewonger, John
Johnson, Teresa R.
Chen, Man
LaVigne, Daaimah
Roberts, Jeffrey N.
Cuburu, Nicolas
Schiller, John T.
Buck, Christopher B.
author_sort Graham, Barney S.
collection PubMed
description Mucosal immunization may be important for protection against pathogens whose transmission and pathogenesis target mucosal tissue. The capsid proteins of human papillomavirus (HPV) confer tropism for basal epithelium and can encapsidate DNA during self-assembly to form pseudovirions (PsV). Therefore, we produced mucosal vaccine vectors by HPV PsV-encapsidation of DNA plasmids expressing an experimental antigen derived from the M and M2 proteins of respiratory syncytial virus. Intravaginal (IVag) delivery elicited local and systemic M/M2-specific CD8+ T-cell and antibody responses in mice comparable to a 10,000-fold higher dose of naked DNA. A single HPV PsV IVag immunization primed for M/M2-specific-IgA in nasal and vaginal secretions. Based on light emission and immunofluorescent microscopy, immunization with HPV PsV-encapsidated luciferase- and red fluorescent protein (RFP)-expressing plasmids resulted in transient antigen expression (<5 days) restricted to the vaginal epithelium. HPV PsV encapsidation of plasmid DNA is a novel strategy for mucosal immunization that may provide new vaccine options for selected mucosal pathogens.
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spelling pubmed-29244642011-03-01 Mucosal Delivery of Human Papillomavirus Pseudovirus-Encapsidated Plasmids Improves Potency of DNA Vaccination Graham, Barney S. Kines, Rhonda Corbett, Kizzmekia S. Nicewonger, John Johnson, Teresa R. Chen, Man LaVigne, Daaimah Roberts, Jeffrey N. Cuburu, Nicolas Schiller, John T. Buck, Christopher B. Mucosal Immunol Article Mucosal immunization may be important for protection against pathogens whose transmission and pathogenesis target mucosal tissue. The capsid proteins of human papillomavirus (HPV) confer tropism for basal epithelium and can encapsidate DNA during self-assembly to form pseudovirions (PsV). Therefore, we produced mucosal vaccine vectors by HPV PsV-encapsidation of DNA plasmids expressing an experimental antigen derived from the M and M2 proteins of respiratory syncytial virus. Intravaginal (IVag) delivery elicited local and systemic M/M2-specific CD8+ T-cell and antibody responses in mice comparable to a 10,000-fold higher dose of naked DNA. A single HPV PsV IVag immunization primed for M/M2-specific-IgA in nasal and vaginal secretions. Based on light emission and immunofluorescent microscopy, immunization with HPV PsV-encapsidated luciferase- and red fluorescent protein (RFP)-expressing plasmids resulted in transient antigen expression (<5 days) restricted to the vaginal epithelium. HPV PsV encapsidation of plasmid DNA is a novel strategy for mucosal immunization that may provide new vaccine options for selected mucosal pathogens. 2010-06-16 2010-09 /pmc/articles/PMC2924464/ /pubmed/20555315 http://dx.doi.org/10.1038/mi.2010.31 Text en http://www.nature.com/authors/editorial_policies/license.html#terms Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms
spellingShingle Article
Graham, Barney S.
Kines, Rhonda
Corbett, Kizzmekia S.
Nicewonger, John
Johnson, Teresa R.
Chen, Man
LaVigne, Daaimah
Roberts, Jeffrey N.
Cuburu, Nicolas
Schiller, John T.
Buck, Christopher B.
Mucosal Delivery of Human Papillomavirus Pseudovirus-Encapsidated Plasmids Improves Potency of DNA Vaccination
title Mucosal Delivery of Human Papillomavirus Pseudovirus-Encapsidated Plasmids Improves Potency of DNA Vaccination
title_full Mucosal Delivery of Human Papillomavirus Pseudovirus-Encapsidated Plasmids Improves Potency of DNA Vaccination
title_fullStr Mucosal Delivery of Human Papillomavirus Pseudovirus-Encapsidated Plasmids Improves Potency of DNA Vaccination
title_full_unstemmed Mucosal Delivery of Human Papillomavirus Pseudovirus-Encapsidated Plasmids Improves Potency of DNA Vaccination
title_short Mucosal Delivery of Human Papillomavirus Pseudovirus-Encapsidated Plasmids Improves Potency of DNA Vaccination
title_sort mucosal delivery of human papillomavirus pseudovirus-encapsidated plasmids improves potency of dna vaccination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2924464/
https://www.ncbi.nlm.nih.gov/pubmed/20555315
http://dx.doi.org/10.1038/mi.2010.31
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